Abstract: Conditions of tissue culture of Taxus chinensis var.Softwood was studied by inducing stem segment with a axillary buds bud to plants directly. The results showed that the medium DCR +6- BA 0.5mg / L + NAA 0.8mg / L was most optimal bud-induced and the medium 1/2MS + N AA 0.1mg / L was root.-induced for Taxus chinensis var.Softwood. Robust seedlings at height about 2cm were moved into the medium DCR + NAA 0.1mg / L to root. Roots grew out about 45 days. when the root length of about 0.5 cm, the seedlings were transplanted into the peat and sand matrix to culture under temperatures 25 ℃, humidity above 70% in 30 days, gradually increasing light intensity, the plant survival rate reached to 57.3%.