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农学学报

农学学报 ›› 2013, Vol. 3 ›› Issue (11): 25-29.

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

春兰SRAP-PCR反应体系的建立和优化

牛田 张林 王厚新 李承秀   

  • 收稿日期:2013-05-14 修回日期:2013-06-19 出版日期:2013-11-20 发布日期:2013-11-20
  • 基金资助:
    国家公益专项“国兰新品种培育与产业化生产关键技术研究”

Establishment and Optimization of SRAP-PCR Reaction System of Cymbidium goeringii

  • Received:2013-05-14 Revised:2013-06-19 Online:2013-11-20 Published:2013-11-20

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摘要： 摘 要：【研究目的】为获得春兰的 SRAP 标记图谱，对春兰SRAP-PCR 反应体系进行了初步研究【方法】通过正交试验设计,从Mg2+、dNTPs、Taq酶、引物、模板5种因素4个水平对春兰SRAP-PCR反应体系进行优化。【结果】所建立的体系为：25μl反应体系中含有2.5mmol/L的Mg2+ ，2mmol/L的dNTP, 0.5U的Taq酶,1.2 μmol/L的引物,90 ng的模板。【结论】为春兰指纹图谱的构建奠定基础。

关键词: 余甘子, 余甘子, 氨基酸, 成分分析

Abstract: Abstract: 【OBJECTIVE】To obtain Sequence-related amplified polymorphism (SRAP) fingerprints of Cymbidium goeringii, the factors influencing the SRAP reaction system were studied【METHOD】The major components of SRAP,such as concentrations of Mg2+,dNTP, Taq DNA polymerase,primers and template,were optimized in this study by orthogonal design in five factors four levels respectively. 【RESULTS】The results showed that the optimum SRAP reaction system included Mg2+ 2.5mmol/L,dNTP 2mmol/L, Taq DNApolymerase 0.5U,primer 1.2mmol/L and DNA template 90 ng in the total 25μL reaction system.【CONCLUSION】Lay the foundation for the construction of fingerprints.

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