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农学学报

农学学报 ›› 2011, Vol. 1 ›› Issue (6): 42-48.

• 土壤肥料 资源环境 生态 • 上一篇    下一篇

增强微流控芯片化学发光检测灵敏度的研究

王修中   

  • 收稿日期:2011-07-21 修回日期:2011-08-04 出版日期:2011-08-15 发布日期:2011-08-15
  • 基金资助:

    芯片电泳和化学发光高性能联用和全分析系统小型化研究;微芯片电泳和化学发光高性能检测联用的研究

Study of Enchancing Detection Sensitivity of Microchip Chemiluminescence System

  • Received:2011-07-21 Revised:2011-08-04 Online:2011-08-15 Published:2011-08-15

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摘要：

本文利用金属离子催化鲁米诺-过氧化氢反应，通过比较几种结构不同的微流控芯片，对影响检测发光信号灵敏度的因素，如试剂浓度、流速和微通道的尺度等作了详细地讨论。提出了具有双螺旋形结构芯片的微流动注射系统，大大增强了化学发光检测的灵敏度。在最佳实验条件下，铬离子（Cr3+）浓度在5.0 × 10 -9 ~ 2.0 × 10 -6 mol/L范围内与其体系的发光信号强度具有一定的线性关系，对浓度为6.0×10?8 mol/L的铬连续11次进样，相对标准偏差为1.59 %，其检出限为1.50×10-9 mol/L。该体系成功地用于天然湖水和自来水中金属铬的分析和检测。

关键词: 雷蒙德氏棉, 雷蒙德氏棉, SWEET基因家族, 染色体定位, 基因结构, 进化分析

Abstract:

Based on the metal ion-catalyzed luminol–peroxide reaction, several different configuration microchip have been compared. Influencing factors of chemiluminescence sigals, such as concentration and flow rate of reagents and scale of microchannels have been discussed. We proposed a new microflow injection system with double spiral channel configuration and the sensitivity was greatly enhanced due to more efficient mixing of the analyte and reagent solutions. Under the optimized conditions, a linear calibration curve (R2 = 0.997) over the range 5.0 × 10-9 to 2.0 × 10-6 mol L-1 was obtained with the detection limit of 1.50×10?9 mol L?1 for the chromium ion. The relative standard deviation was found to be 1.59 % (n = 11) for 6.0×10?8 mol L?1. The proposed method has been applied successfully to the analysis of natural lake water and tap water.