超高效液相色谱-串联质谱法在农药残留检测中的应用

doi:10.11923/j.issn.2095-4050.cjas18040026

农学学报 ›› 2019, Vol. 9 ›› Issue (6): 51-56.doi: 10.11923/j.issn.2095-4050.cjas18040026

所属专题：植物保护

• 农业工程农业机械生物技术食品科学 • 上一篇下一篇

超高效液相色谱-串联质谱法在农药残留检测中的应用

蒋剑¹, 靳艳玲², 马丽³

1.广电计量检测成都有限公司;2.中国科学院成都生物研究所;3.四川出入境检验检疫局

收稿日期:2018-04-25 修回日期:2018-07-09 接受日期:2018-07-17 出版日期:2019-07-01 发布日期:2019-07-01
通讯作者: 靳艳玲 E-mail:jinyl@cib.ac.cn
基金资助:
现代农业产业技术体系建设专项“质量安全与营养品质评价”(CARS-10-B-22)。

Application of Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry in Pesticide Residue Detection

Received:2018-04-25 Revised:2018-07-09 Accepted:2018-07-17 Online:2019-07-01 Published:2019-07-01

摘要/Abstract

摘要： 以超高效液相色谱-串联质谱法检测多种农药残留的基本要素作为切入点，归纳了近年来超高效液相色谱-串联质谱利用技术的研究进展，对不同基质样品开展多种农残检测时所采取的萃取工艺、净化工艺、色谱柱的选择、质谱类型、离子源模式进行了分析，认为生物样品成分复杂、内源性物质会对目标物质的测定造成干扰，根据生物样品的特点针对性地开展前处理并消减基质效应对于提升结果的准确性具有重要意义，同时还提出了建立超高效液相色谱-串联质谱方法时需要考虑的主要问题。

关键词: 赶黄草, 赶黄草, CNKI, 文献分析, 专题分析

Abstract: According to the basic influence factors of multi-pesticide residues detection by Ultra High Performance Liquid Chromatography (UPLC)- tandem Mass Spectrometry (MS/MS), the key points of literatures in recent years about pesticide residues detection by UPLC-MS/MS were summarized, including extraction technology, purification process, selection of chromatographic column, type of mass spectrometry and ion source model for samples of different matrix. It was considered that the pretreatment of biological samples and removal of matrix effects had important effects on the accuracy of the results, because biological samples were usually complex and the endogenous substances would interfere with the determination of the target substance. Then, the main factors that need to be considered in establishment of UPLC-MS/MS were proposed.

中图分类号:

S481

蒋剑,靳艳玲,马丽. 超高效液相色谱-串联质谱法在农药残留检测中的应用[J]. 农学学报, 2019, 9(6): 51-56.

参考文献

[1] Tabrez S, Sayyed H. Ultra high performance liquid chromatography (UPLC):a new trend in analysis[J].World Journal of Pharmaceutical Research,2016,5(3):387- 394.
[2] 张帅,丛海林,于冰.超高效液相色谱的发展及在分析领域的应用[J].分析仪器, 2017(6):16-27.
[3] 安蓉,薄美萍.超高效液相色谱(UPLCTM)与质谱联用技术改善药物和代谢分析的结果质量[J].现代科学仪器, 2006,1:20-23.
[4] 郭智,张煊,宋冬梅,等.液相色谱-串联质谱法在药物代谢研究中应用最新进展[J].中国新药杂志,2007,16(23):1981-1985.
[5] 吴春红,聂继云,徐国锋,等.果蔬中农药残留检测技术研究进展[J].植物保护,2009, 35(3):23-26.
[6] 刘士华,王兴征,侯淑云,等.农药残留检测前处理步骤及其质量控制[J].北京农业,2014,15:5-6.
[7] Elsa T R, Miguel A P, Noelia S G,et al.A single-step pesticide extraction and clean-up multi-residue analytical method by selective pressurized liquid extraction followed by on-line solid phase extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry for complex matrices[J].Journal of Chromatography A, 2016,1452:10-17.
[8] Chen X X, Dong F S. Xu J, et al.Effective Monitoring of Fluxapyroxad and Its Three Biologically Active Metabolites in Vegetables, Fruits, and Cereals by Optimized QuEChERS Treatment Based onSUPLC-MS/MS[J].Journal of Agricultural and Food Chemistry,2016,64(46):S8935-8943.
[9] Zywitz D, Anastassiades M, Scherbaum E. Simultaneous determination of macrocyclic lactone insecticides in fruits and vegetables using LC-MS/MS[J].Deutsche lebensmittel-rundschau.2003,100(4):140-150
[10] 冯楠,路勇,姜洁,等.QuEChERS‐超高效液相色谱串联质谱法快速筛查食品中73种有毒有害物质[J].食品科学,2013,34(16):221-227.
[11] 鲁红,吴国杰,梁世强,等.QuEChERS/超高效液相色谱-高分辨飞行时间质谱联用法测定蔬菜中4种有机磷农药残留[J].广东农业科学,2012,39(11):181-184.
[12] 郭虹,任武洁,魏玉霞,等.分散固相萃取-超高效液相色谱-串联质谱法测定蔬菜中7种植物生长调节剂[J].中国卫生检验杂志,2018, 28 (6 ) :660-666.
[13] Anastassiades M,Kolberg D I, Benkenstein A, et al. Quick Method for the Analysis of numerous Highly Polar Pesticides in Foods of Plant Origin via LC-MS/MS involving Simultaneous Extraction with Methanol (QuPPe-Method)[S].EU Reference Laboratories for Residues of Pesticides Single Residue Methods,Version 9.3 ,2017.
[14] Anastassiades M,Kolberg D I, Benkenstein A, et al.Quick Method for the Analysis of Residues of numerous Highly Polar Pesticides in Food Commodities involving Simultaneous Extraction with Methanol and Determination via LC-MS/MS (QuPPe-AO-Method) II. Food of Animal Origin [S].EU Reference Laboratories for Residues of Pesticides Single Residue Methods,Version 2,2016.
[15] 吴延灿,蒋冰心,施艳红,等.QuPPe‐超高效液相色谱串联质谱法测定蔬菜中呋虫胺及其代谢物残留[J].食品科学,2018,1:1-8.
[16] 纪律,徐峻卿,李启,等.快速溶剂萃取－超高效液相色谱串联质谱法测定竹笋中的百草枯残留[J].中国卫生检验杂志, 2018,28 (1) :18-21.
[17] YoshiyukiSS,TakaomiST,TakahiroSD,et al.Analysis of Iodothyronines in Dietary Supplements Containing Dried Thyroid by HPLC Using a Pre-column Derivative[J]. Food Analytical Methods,2012,5(5):1033-1039.
[18] 王永莲,刘刚,周凯,等.超高效液相色谱串联质谱法测定白菜、青椒和葡萄中的仲丁胺[J].农产品质量与安全,2018, 1 :75-79.
[19] 陈晓英,林钦,戴明.超高效液相色谱-串联质谱法测定粮谷中18种农药残留[J].农药, 2018, 57(3): 196-199.
[20] 户江涛.超高效液相色谱－串联质谱法测定大豆中草甘膦及其代谢物氨甲基膦酸的残留[J].分析仪器,2018,1:131-135.
[21] Shi Z L, Zhang S L, Huai Q R, et al.Methylamine-modified graphene-based solid phase extraction combined with UPLC-MS/MS for the analysis of neonicotinoid insecticides in sunflower seeds[J].Talanta,2017,162:300-308.
[22] Lehmann E,Oltramare C, De L A, et al.Development of a modified QuEChERS method for multi-class pesticide analysis in human hair by GC-MS and UPLC-MS/MS[J].Analytica Chimica Acta,2018, 999:87-98.
[23] 刘慧慧,张华威,魏潇,等.超高效液相色谱-串联质谱法测定水产品中13种磺酰脲类除草剂残留量[J].分析化学,2018,3:386-392.
[24] 张乐,刘超,翁丽萍.超高效液相色谱-四级杆-静电场轨道阱高分辨质谱筛查水产品中62种药物残留[J].浙江农业科学,2018,59(4):617-620.
[25] Hou R Y, Jiao W T, Xiao Y,et al.Novel use of PVPP in a modified QuEChERS extraction method for UPLC-MS/MS analysis of neonicotinoid insecticides in tea matrices[J].Analytical Methods, 2015, 7:5521-5529.
[26] Zhao H X,Zhao S C, Deng L G, et al.Rapid Determination of Organonitrogen, Organophosphorus and Carbamate Pesticides in Tea by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)[J].Food Analytical Methods,2013,6:497-505.
[27] 余巍,刘军义,汪文龙,等.超快速液相色谱-四极杆/静电场轨道阱高分辨质谱快速筛查淀粉中的 224 种农药残留[J].环境化学,2014,33(12):2225-2227.
[28] Mao B P, Jia Z W, Mao X H,et al.Determination of triazine residues in herbal plants using molecularly imprinted polymer extraction followed by UPLC-MS/MS[J].AnalyticalSMethods, 2013, 5, 3582-3590.
[29] Chen L N,Song F R,Liu Z Q,et al.Study of the ESI and APCI interfaces for the UPLC–MS/MS analysis of pesticides in traditional Chinese herbal medicine[J]. Analytical and Bioanalytical Chemistry,2014,406:1481-1491.
[30] 陈永艳,吕佳,韩嘉艺,等.直接进样－超高效液相色谱－串联质谱法测定饮用水中莠去津及其4种代谢产物[J].中国卫生检验杂志, 2016,26(21 ):3069-3071.
[31] 庄乾坤,郑和辉,卞战强,等.超高效液相色谱－串联质谱法测定饮用水中6种三唑类杀菌剂[J].中国卫生检验杂志,2016, 26(2):153-155.
[32] Lu N, Wang T, Zhao P, Zhang L J,et al.Experimental and molecular docking investigation on metal-organic framework MIL-101(Cr) as a sorbent for vortex assisted dispersive micr-solid-phase extraction of trace 5-nitroimidazole residues in environmental water samples prior to UPLC-MS/MS analysis[J].Analytical and Bioanalytical Chemistry,2016,408:8515-8528.
[33] 金晶,顾海东,高仕谦,等.超高效液相色谱-串联质谱法测定水中4种苯脲类除草剂的含量[J].理化检验(化学分册),2018,56(1):77-81.
[34] Quinete N ,Wang J, Fernandez A,et al.Outcompeting GC for the detection of legacy chlorinated pesticides: online-SPE UPLC APCI/MSMS detection of endosulfans at part per trillion levels[J].Analytical and Bioanalytical Chemistry,2016,405:5887-5899.
[35] Singh S P, Dwivedi N, Raju K S,et al.Validation of a Rapid and Sensitive UPLC–MS-MS Method Coupled with Protein Precipitation for the Simultaneous Determination of Seven Pyrethroids in 100 μL of Rat Plasma by Using Ammonium Adduct as Precursor Ion[J].Journal of Analytical Toxicology, 2016;40:213-221.
[36] 郭震,常靖,杨瑞琴,等.超高效液相色谱－四极杆－飞行时间质谱同时筛查腐败血液中24种农药[J].分析测试学报,2015,34(9):986-992.
[37] Kalogridi E C,Christophoridis C,Bizani E,et al.Part I: Temporal and spatial distribution of multiclass pesticide residues in lake waters of Northern Greece: application of an optimized SPE-UPLC-MS/MS pretreatment and analytical method[J].EnvironmentalSScienceSand PollutionSResearch,2014,21:7239-7251.
[38] Cheng X L,Qi L W,Wang Q,et al.Highly efficient sample preparation and quantification of constituents from traditional Chinese herbal medicines using matrix solid-phase dispersion extraction and UPLC-MS/MS[J].Analyst, 2013, 138, 2279-2288.
[39] Song Y, Guo Y Z, Zhang X,et al.Synthesis of Isotopically Labeled 13C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil[J].Molecules,2016, 21:89-97.
[40] 徐国宾.飞行时间质谱及串联质谱关键技术的系统研究[D].复旦大学,2010.
[41] Bauer A,Luetjohann JS,Hanschen, F S, et al.Identification and characterization ofSpesticideSmetabolites in Brassica species by liquid chromatography travelling wave ion mobility quadrupole time-of-flight mass spectrometry (UPLC-TWIMS-QTOF -MS)[J].Food Chemistry,2018,244:292-303.
[42] Sivaperumal P, Salauddin A,Kumar A R,et al.Determination ofSPesticideSResidues in Mango Matrices by Ultra High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry[J].Food Analytical Methods,2017,10(7):2346-2357.

超高效液相色谱-串联质谱法在农药残留检测中的应用

Application of Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry in Pesticide Residue Detection

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们