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农学学报

农学学报 ›› 2011, Vol. 1 ›› Issue (4): 48-51.

所属专题： 生物技术

• 畜牧 兽医 水产 • 上一篇    下一篇

紫薄鳅染色体核型分析

孟妍 方翠云 祖国掌 唐燕高 徐大宝 洪斌   

  • 收稿日期:2011-04-18 修回日期:2011-06-01 出版日期:2011-06-15 发布日期:2011-06-15

A Preliminary Study on Chromosome Preparaation and Karytype of Leptobotia taeniops

  • Received:2011-04-18 Revised:2011-06-01 Online:2011-06-15 Published:2011-06-15

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摘要：

为研究分析长江铜陵地区紫薄鳅染色体的数目及核型，以紫薄鳅头肾为材料，采用植物血凝素(PHA)和秋水仙素体内直接注射培养，空气干燥法制片制备紫薄鳅染色体标本，对紫薄鳅的染色体核型进行分析。核型分析的结果表明：紫薄鳅二倍体染色体数目为2n=50，核型公式为：6m+10sm+8st+26t，臂数为66。研究结果丰富了紫薄鳅细胞遗传学参数，为紫薄鳅杂交育种提供理论依据。

关键词: 体制创新, 体制创新

Abstract:

To analysis the chromosome number and karyotype of Leptobotia taeniops. The karyotype of Leptobotia taeniops was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines and used air drying method to make chromosome samples. The total chromosome number of the fish was 50, the karyotype formula was 2n=6m+10sm+8st+26t, and NF=66. There was no visible evidence of po1yploidy chromosome and sex chromosome. This study provided Leptobotia taeniops with cytogenetics paramete, and offered a theoretical basis for Cross breeding of Leptobotia taeniops.

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