为探析不同湿度环境下香蕉苗对低温的响应及对香蕉苗实际育苗生产提供抵御低温的技术支持，对不同湿度环境下香蕉苗进行梯度低温处理。结果表明，形态上，同一低温处理时高湿度（RH>90%）下香蕉苗受冷害程度明显弱于中湿度（60%~70%）下的香蕉苗；6oC低温高湿度下具有近90%的组织含水量，而中湿度下含水量仅为62.15%，严重失水导致萎蔫，在低温处理时高湿度下抗失水能力强于中湿度；低温胁迫下高湿度环境能减缓细胞膜渗漏率、丙二醛的增加，6℃低温导致中湿度下MDA高出高湿度下24.40%，表明高湿度下低温处理对细胞膜的伤害弱于中湿度下的处理。

For studying banana response on low temperature at high and middle humidity level, and for providing technology service for banana plantlet production when chilling coming. Effect of low temperature treatments on banana plantlets grown under different humidity was tested. The results showed that chilling injury to banana plantlets in the high humidity (RH>90%) condition was significantly lighter than in the middle humidity (60%-70%) condition under the same low temperatures. Under high humidity, the plantlets have 90% higher water content and strong dehydration resistance compared with the middle humidity, about 62.15% water content and fade when 6℃ chilling reaches. The physiology parameter, rate of cell membrane leakage, MDA of plantlet treated was tested, and the results showed that 6oC chilling at middle humidity lead to the 24.40% higher MDA content than high humidity, and high humidity can delay the increase of membrane leakage rate, MDA content when treated with chilling, and chilling injury on cell membrane in high humidity was gentler than it in middle humidity.