明确温度、培养方式、盐分胁迫对何首乌种子发芽的影响，可以为何首乌种子发芽率检验及田间播种提供参考。采用培养皿法培养种子，统计发芽率。结果表明，何首乌种子在25℃时，发芽率最高；在盐胁迫条件下，随着盐浓度的升高，种子发芽率呈下降趋势，低盐条件下，发芽率与对照差异不显著；在同一温度条件下，暗培养比光培养发芽率高，纸间比纸上发芽率高。何首乌种子发芽率检验时，应选择在25℃，无光照条件下，双层滤纸间培养，在发芽试验第9天，作为计算种子发芽势的天数。播种育苗田盐分浓度不能高于3 g/L，过高则影响何首乌种子发芽。

To study the influence of the seed germination of Polygonum multiflorum by different temperatures, cultivating methods and salt stresses, which can provide reference for seed germination inspection and field seeding. The author took petri dishes to culture seeds and counted the germination rate. The results showed that the highest germination rate occured at 25℃; in salt-stress condition, with the increase of the salt concentration seed germination rate declined and under the condition of low salt concentration germination rate showed no obvious difference with the controlling group; at the same temperature, germination rate was higher when cultivated with dark than under light, and seeds cultured in the filters had higher germination rate than those which cultured on the filter. Germination experiment should carry out at 25℃, with light and between paper. Seeds germinate potential should calculate after the germination experiment progressing 9 days. When planting, salt concentration of the planting seedling field could not above 3 g/L, otherwise the germination would be affected.