通过设计正交试验及设置光照条件等单因素试验，探讨不同种类、不同浓度的植物生长激素、不同光照强度对桑组培苗继代增殖的影响情况。结果表明，桑组培苗继代增殖的最佳生长激素配比配方为：6-BA 1.0 mg/L+IBA 0.1 mg/L；最佳培养条件为：温度27℃，光照强度2000~2500 lx，先暗培养5天，后光照时间14 h/d，此条件下发芽率为100%。由此可见过高的细胞分裂素和生长素会对组培苗产生不利影响；初期先适量暗培养，发芽率高于直接光照培养，并且能有限减弱褐化现象，同时还能降低生产成本。

Different kinds and concentrations of plant growth hormone and different light intensity on sub-proliferation of mulberry tissue culture were studied. Through orthogonal experiment design and setting light condition experiment to evaluate the effects of sub-proliferation of mulberry tissue culture at different factors. Results showed that the best growth hormone ratio formula were 6-BA 1.0 mg/L+IBA 0.1 mg/L; the best culture conditions were temperature 27℃, light intensity 2000-2500 lx, first dark training 5 days, light period 14 h/d, the germination rate were 100% under this condition. High cytokinins and auxin would produce adverse effect on tissue culture seedling. The initial dark training could improve germination rate, and limited abate browning phenomenon, at the same time also can reduce the production cost.