以‘金荷鼎’ב赤蕙’根状茎为材料,研究根状茎的增殖情况,包括基本培养基的筛选、6-BA与NAA的浓度组合对增殖的影响。不同培养基对根状茎生长情况有明显影响。1/2MS培养基为春兰根状茎的基本培养基,1/2MS+NAA 1 mg/L+6-BA 0.5 mg/L为春兰根状茎的最佳增殖培养基,增殖倍数达3.00。杂交育种技术结合组织培养是国兰新品种选育的有效途径。
Taking ‘Jinheding’ and ‘Chihui’ as parents, rootstocks were used as materials for studying the multiplication of rootstocks. There were including choices of basic medium, the effects of 6-BA and NAA on the multiplication. The result showed that the growth state was affected evidently by medium. 1/2MS medium was the basic medium for rootstocks of Cymbidium goeringii. 1/2MS+ NAA 1 mg/L+6-BA 0.5 mg/L was the best medium for multiplication. proliferation rate was 3.00. Cross breeding technology combining with tissue culture was the effective way for the new species breeding of Cymbidium.
牛田 张林 李承秀 孙芳 于永畅 颜婷美 王长宪 .春兰‘金荷鼎’杂交后代离体快繁的研究.农学学报 ,2013,3(3): 44-46