克氏原螯虾促雄性腺摘除前后血淋巴外泌体差异蛋白组学分析

doi:10.11923/j.issn.2095-4050.cjas2023-0220

农学学报 ›› 2024, Vol. 14 ›› Issue (11): 56-63.doi: 10.11923/j.issn.2095-4050.cjas2023-0220

克氏原螯虾促雄性腺摘除前后血淋巴外泌体差异蛋白组学分析

杨思琦(), 王齐帅, 李艳和()

华中农业大学水产学院/长江经济带大宗水生生物产业绿色发展教育部工程研究中心/湖北洪山实验室，武汉 430070

收稿日期:2023-09-09 修回日期:2023-11-21 出版日期:2024-11-19 发布日期:2024-11-19
通讯作者:
李艳和，女，1976年出生，安徽滁州人，博士，副教授，主要从事水产动物遗传与育种研究。通信地址：430070 湖北省武汉市洪山区狮子山街1号华中农业大学，E-mail：liyanhe@mail.hzau.edu.cn。
作者简介:
杨思琦，女，1998年出生，四川西昌人，硕士，研究方向：水产动物遗传与育种。通信地址：430070 湖北省武汉市洪山区狮子山街1号华中农业大学，E-mail：Yangsiqi@webmail.hzau.edu.cn。
基金资助:
中央高校基本科研业务费专项资金资助项目(2662020SCPY004); 湖北省重点研发计划项目“小龙虾优良品系（种）培育及其关键技术研究”(2021BBA232)

Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation

YANG Siqi(), WANG Qishuai, LI Yanhe()

Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education/ Hubei Hongshan laboratory /College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China

Received:2023-09-09 Revised:2023-11-21 Online:2024-11-19 Published:2024-11-19

摘要/Abstract

摘要：

为了了解可能参与克氏原螯虾性腺分化的相关调控因子，对克氏原螯虾促雄性腺摘除前后血淋巴外泌体进行了蛋白组测序和分析。结果表明，在摘除促雄性腺前后血淋巴外泌体中一共鉴定到蛋白质469个，差异表达蛋白98个。14-3-3 zeta和cell division cycle protein 27 homolog这2个差异表达蛋白可能参与了克氏原螯虾的性别调控。

关键词: 克氏原螯虾, 促雄性腺, 外泌体, 蛋白组

Abstract:

In order to understand the regulation factors that may be involved in gonadal differentiation of P. Procambarus, in this study, the proteome of exosomes in the hemolymph before and after androgenic gland ablation of P. clarkii was sequenced and analyzed. The results showed that a total of 469 proteins were identified in hemolymph exosomes, and 98 differentially expressed proteins were screened. Among which two differentially expressed proteins, 14-3-3 zeta and cell division cycle protein 27 homolog, may be involved in the sex regulation of P. clarkii.

Key words: Procambarus clarkii, androgenic gland, exosomes, proteome

杨思琦, 王齐帅, 李艳和. 克氏原螯虾促雄性腺摘除前后血淋巴外泌体差异蛋白组学分析[J]. 农学学报, 2024, 14(11): 56-63.

YANG Siqi, WANG Qishuai, LI Yanhe. Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation[J]. Journal of Agriculture, 2024, 14(11): 56-63.

图/表 7

参考文献 26

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蛋白ID	蛋白名称	序列长度/aa	备注
TRINITY_DN46360_c2_g2	Vitellogenin（卵黄蛋白原）	1592	H组有， Q组没有的蛋白
TRINITY_DN45848_c0_g1	Neural-cadherin（神经钙粘蛋白）	3003	H组有， Q组没有的蛋白
TRINITY_DN36934_c0_g1	Myosin-8（肌球蛋白-8）	1002	H组有， Q组没有的蛋白
TRINITY_DN31439_c0_g3	Teneurin-m（跨膜蛋白-m）	2552
TRINITY_DN39238_c5_g6	Apolipophorins（载脂蛋白）	1771
TRINITY_DN41980_c0_g3	Glycogen debranching enzyme（糖原脱支酶）	1632
TRINITY_DN46185_c1_g1	Twitchin（颤搐蛋白）	6121	Q组有， H组没有的蛋白
TRINITY_DN39783_c1_g2	Zinc finger homeobox protein 3（锌指同源盒蛋白3）	2320
TRINITY_DN30023_c2_g2	Zinc finger protein 423 homolog（锌指蛋白423同源物）	1171
TRINITY_DN33875_c0_g2	Agrin（集聚蛋白）	1575
TRINITY_DN39770_c1_g1	Histone-lysine N-methyltransferase SETD2（组蛋白赖氨酸N甲基转移酶SETD2）	1105
TRINITY_DN35993_c1_g1	RB1-inducible coiled-coil protein 1（RB1感应线圈蛋白1）	1464

蛋白ID	蛋白名称	序列长度/aa	备注
TRINITY_DN46360_c2_g2	Vitellogenin（卵黄蛋白原）	1592	H组有， Q组没有的蛋白
TRINITY_DN45848_c0_g1	Neural-cadherin（神经钙粘蛋白）	3003	H组有， Q组没有的蛋白
TRINITY_DN36934_c0_g1	Myosin-8（肌球蛋白-8）	1002	H组有， Q组没有的蛋白
TRINITY_DN31439_c0_g3	Teneurin-m（跨膜蛋白-m）	2552
TRINITY_DN39238_c5_g6	Apolipophorins（载脂蛋白）	1771
TRINITY_DN41980_c0_g3	Glycogen debranching enzyme（糖原脱支酶）	1632
TRINITY_DN46185_c1_g1	Twitchin（颤搐蛋白）	6121	Q组有， H组没有的蛋白
TRINITY_DN39783_c1_g2	Zinc finger homeobox protein 3（锌指同源盒蛋白3）	2320
TRINITY_DN30023_c2_g2	Zinc finger protein 423 homolog（锌指蛋白423同源物）	1171
TRINITY_DN33875_c0_g2	Agrin（集聚蛋白）	1575
TRINITY_DN39770_c1_g1	Histone-lysine N-methyltransferase SETD2（组蛋白赖氨酸N甲基转移酶SETD2）	1105
TRINITY_DN35993_c1_g1	RB1-inducible coiled-coil protein 1（RB1感应线圈蛋白1）	1464

编号	描述	分类
K16197/K03350	Oocyte meiosis（卵母细胞减数分裂）	KEGG通路
K03350	Progesterone-mediated oocyte maturation（孕激素介导的卵母细胞成熟）	KEGG通路
GO:0008585	Female gonad development（雌性性腺发育）	GO term
GO:0046545	Development of primary female sexual characteristics（初级雌性性征发育）
GO:0008406	Gonad development（性腺发育）
GO:0007548	Sex differentiation（性分化）
GO:0045137	Development of primary sexual characteristics（初级性征的发展）
GO:0046660	Female sex differentiation（雌性性分化）
GO:0007565	Female pregnancy（雌性怀孕）
GO:0048608	Reproductive structure development（生殖结构发育）
GO:0061458	Reproductive system development（生殖系统发育）
GO:0008103	Oocyte microtubule cytoskeleton polarization（卵母细胞微管细胞骨架极化）
GO:0060180	Female mating behavior（雌性交配行为）
GO:0007309	Oocyte axis specification（卵母细胞轴规格化）
GO:0007308	Oocyte construction（卵母细胞的构建）
GO:0001541	Ovarian follicle development（卵泡发育）
GO:0030716	Oocyte fate determination（卵母细胞命运决定）
GO:2000242	Negative regulation of reproductive process（生殖过程的负调控）
GO:0048599	Oocyte development（卵母细胞发育）
GO:0035112	Genitalia morphogenesis（生殖器形态发生）
GO:0009994	Oocyte differentiation（卵母细胞分化）
GO:0046661	Male sex differentiation（雄性性分化）
GO:0090598	Male anatomical structure morphogenesis（雄性解剖结构的形态发生）
GO:0007314	Oocyte anterior/posterior axis specification（卵母细胞前/后轴规范化）
GO:0046546	Development of primary male sexual characteristics（初级雄性性征的发展）
GO:0032355	Response to estradiol（对雌二醇的反应）
GO:0048515	Spermatid differentiation（精细胞分化）
GO:0019098	Reproductive behavior（生殖行为）
GO:0007283	Spermatogenesis（精子生成）
GO:0045172	Germline ring canal（生殖系环管）

编号	描述	分类
K16197/K03350	Oocyte meiosis（卵母细胞减数分裂）	KEGG通路
K03350	Progesterone-mediated oocyte maturation（孕激素介导的卵母细胞成熟）	KEGG通路
GO:0008585	Female gonad development（雌性性腺发育）	GO term
GO:0046545	Development of primary female sexual characteristics（初级雌性性征发育）
GO:0008406	Gonad development（性腺发育）
GO:0007548	Sex differentiation（性分化）
GO:0045137	Development of primary sexual characteristics（初级性征的发展）
GO:0046660	Female sex differentiation（雌性性分化）
GO:0007565	Female pregnancy（雌性怀孕）
GO:0048608	Reproductive structure development（生殖结构发育）
GO:0061458	Reproductive system development（生殖系统发育）
GO:0008103	Oocyte microtubule cytoskeleton polarization（卵母细胞微管细胞骨架极化）
GO:0060180	Female mating behavior（雌性交配行为）
GO:0007309	Oocyte axis specification（卵母细胞轴规格化）
GO:0007308	Oocyte construction（卵母细胞的构建）
GO:0001541	Ovarian follicle development（卵泡发育）
GO:0030716	Oocyte fate determination（卵母细胞命运决定）
GO:2000242	Negative regulation of reproductive process（生殖过程的负调控）
GO:0048599	Oocyte development（卵母细胞发育）
GO:0035112	Genitalia morphogenesis（生殖器形态发生）
GO:0009994	Oocyte differentiation（卵母细胞分化）
GO:0046661	Male sex differentiation（雄性性分化）
GO:0090598	Male anatomical structure morphogenesis（雄性解剖结构的形态发生）
GO:0007314	Oocyte anterior/posterior axis specification（卵母细胞前/后轴规范化）
GO:0046546	Development of primary male sexual characteristics（初级雄性性征的发展）
GO:0032355	Response to estradiol（对雌二醇的反应）
GO:0048515	Spermatid differentiation（精细胞分化）
GO:0019098	Reproductive behavior（生殖行为）
GO:0007283	Spermatogenesis（精子生成）
GO:0045172	Germline ring canal（生殖系环管）

蛋白ID	蛋白名称	序列长度/aa	分子量/ kDa	非标记定量信号强度-Q	非标记定量信号强度-H	Ratio(H/Q)	表达量上调或下调
TRINITY_DN31302_c2_g2	14-3-3 zeta	206	23.527	28304000	107000000	3.780384398	上调
TRINITY_DN37497_c4_g2	Cell division cycle protein 27 homolog	806	89.33	8123000000	4587100000	0.564705158	下调

克氏原螯虾促雄性腺摘除前后血淋巴外泌体差异蛋白组学分析

Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation

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蛋白ID	String ID	首选名称	注释	表达量上调或下调
TRINITY_DN37497_c4_g2	7955.ENSDARP00000113979	cdc27	Cell division cycle 27	下调
TRINITY_DN36482_c3_g4	7955.ENSDARP00000089879	ubb	Polyubiquitin-c; Ubiquitin C	下调
TRINITY_DN47205_c4_g1	7955.ENSDARP00000063799	gapdh	甘油醛-3-磷酸脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase)；同时具有甘油醛-3-磷酸脱氢酶和亚硝化酶的活性，从而分别在糖酵解和核功能中起作用。甘油醛-3-磷酸脱氢酶是糖酵解中的一个关键酶，还参与核事件，包括转录、RNA运输、DNA复制和细胞凋亡。	上调
TRINITY_DN31218_c2_g2	7955.ENSDARP00000122263	actb2	Actin, cytoplasmic 2；肌动蛋白(Actins)是高度保守的蛋白质，参与各种类型的细胞运动，在所有真核细胞中普遍表达。	上调
TRINITY_DN34937_c1_g1	7955.ENSDARP00000013402	cat	过氧化氢酶(Catalase)；存在于几乎所有需氧呼吸的生物体中，用于保护细胞免受过氧化氢的毒性影响。	上调
TRINITY_DN33274_c1_g3	7955.ENSDARP00000042199	aldoab	果糖二磷酸醛缩酶，第一类(Fructose-bisphosphate aldolase, class i)；属于第一类果糖二磷酸醛缩酶家族	上调