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农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (4): 50-55.

所属专题： 生物技术

• 畜牧 兽医 水产 • 上一篇    下一篇

表皮干细胞标记物在仿刺参正常和再生体壁中的表达研究

李霞 王微微 李强 秦艳杰   

  • 收稿日期:2011-10-17 修回日期:2012-01-05 出版日期:2012-04-20 发布日期:2012-04-20
  • 基金资助:

    辽宁省教育厅创新团队项目;辽宁省教育厅重点实验室项目

Studies on the expression of identification makers of epidermal stem cell in the natural and regenerate body wall of Apostichopus japonicus

  • Received:2011-10-17 Revised:2012-01-05 Online:2012-04-20 Published:2012-04-20

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摘要：

为探究仿刺参表皮再生的机制和再生修复过程中的表皮细胞来源，采用免疫荧光方法，观察β1整合素，α6整合素，角蛋白19 (ck19) 3种表皮干细胞标记物在仿刺参正常及创伤后再生不同时间段的体壁中的表达情况。结果表明ck19在对照组和试验组仿刺参体壁中均呈阳性，并且随着再生的进行，阳性细胞数量和分布区域均有变化。创伤第1天阳性细胞向创伤面上缘迁移，细胞数量同对照组；第3天阳性细胞数量显著下降；第5天阳性细胞数量最多；第7天阳性细胞数量有所下降；11天、13天阳性细胞的数量较第7天稍有增加；第19天阳性细胞数量同对照组，但排列很分散。β1整合素和α6整合素在正常及创伤后再生的体壁中均未见阳性信号出现。结合石蜡切片观察结果进行比较分析认为被ck19标记的细胞是参与仿刺参体壁表皮再生的干细胞和短暂扩增细胞，仿刺参表皮再生的机制属于新建再生。

关键词: 中国对虾, 中国对虾, 哈维氏弧菌, Biolog, 16S rRNA, gyrB, HSP60

Abstract:

In order to study the mechanism of epidermis regeneration in A. japonicus, and the origination of epidermal cells.The expressions of identification markers of stem cells, the β1 integin, α6 integin and keratin 19(ck19), were studied in the natural and regenerate body wall of sea cucumber through Immunofluorescence method. Results showed that ck19 expression was positive both in the control group and the experimental group. At 1 day of wounding, the cells which expressed ck19 moved towards the edges of the wound surface, and the number of these kinds of cells were similar to those in the control group. At 3 days of wounding, the number of cells positively expressed ck19 decreased significantly compared with those in control. At day 5, the number of the positive cells showed the peak value, and then reduced at 7 days of wounding. The numbers of cells which expressed ck19 showed a kind of increasing at 11 and 13 days compared with those at 7 days. And at day 19, the positive cells were disperse, and the number of them showed no significant differences with those in control.β1 integin and α6 integin were not expressed both in wounding and control body wall of sea cucumber. These data, which combined with the results of histochemical, suggested that the cells labeled with ck19 were the epidermal stem cells and the short-term expanded cells, and they involved in the regeneration of epidermis of body wall. The mechanism of epidermis regeneration in A. japonicus is the Epimorphosis.