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农学学报

农学学报 ›› 2014, Vol. 4 ›› Issue (4): 5-8.

所属专题： 小麦

• 农艺科学 作物遗传育种 生理生化 • 上一篇    下一篇

小麦结实小穗数QTL分析

马昊翔 董凡凡 梁增浩 王曙光 王绘艳 景蕊莲 孙黛珍   

  • 收稿日期:2013-09-06 修回日期:2013-10-04 出版日期:2014-04-20 发布日期:2014-04-20
  • 基金资助:
    山西省回国留学人员科研资助项目;CGIAR全球挑战计划;教育部博导类联合项目

QTL Analysis of Fertile Spike Number in Wheat

  • Received:2013-09-06 Revised:2013-10-04 Online:2014-04-20 Published:2014-04-20

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摘要： 为利用分子标记辅助选择技术选育抗旱高产小麦品种提供基础，以小麦旱选10号/鲁麦14 DH群体为材料，在干旱胁迫及正常灌溉两种水分条件下于2010年和2011年考察小麦主茎结实小穗数，通过采用基于混合线性模型的复合区间作图法分析其QTL，研究控制小麦结实小穗数的数量性状基因。共检测到7个加性QTLs和2对上位性QTLs。加性QTL的LOD值介于2.56~5.08之间，分别位于2D、4A、6A和6B染色体上，对表型变异的贡献率为7.19%~13.65%；上位性QTL的LOD值分别为5.24和5.47，分别位于3A和6B与4B和5B染色体上，对表型贡献率分别为13.71%和17.93%。其中QFns-6A-2于2010年正常灌溉和2011年两种水分条件下均被检测到，可用于分子标记辅助育种。

关键词: 夏黑葡萄, 夏黑葡萄, 不同树形, 花序整形, 果实品质

Abstract: In order to provide a basis of breeding drought-resistant and high-yielding wheat varieties using marker-assisted selection technique, fertile spikelet number of main stem was investigated in a doubled haploid population derived from two common wheat varieties ‘Hanxuan 10’ and ‘Lumai 14’ in drought stress and normal irrigation conditions in 2010 and 2011 for analyzing quantitative trait loci (QTL) using composite interval mapping method based on mixed linear model. Seven addictive QTLs for fertile spikelet number with LOD scores ranging from 2.56 to 5.08 were detected on 2D, 4A, 6A, and 6B chromosomes respectively, and the phenotypic variations explained by each QTL ranged from 7.19% to 13.65%. Two pairs of epistatic QTLs with LOD scores of 5.24 and 5.47 were detected on 3A-6B and 4B-5B chromosomes, and the phenotypic variations explained by each pair of epistatic QTLs were 13.71% and 17.93%. Among QTLs detected the QFns-6A-2 was detected under normal irrigation condition in 2010 and under two water regimes in 2011, and can be used in marker-assisted breeding.