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农学学报 ›› 2014, Vol. 4 ›› Issue (4): 83-85.

• 农业工程 农业机械 生物技术 食品科学 • 上一篇    下一篇

低毒高效的植物石蜡切片方法

李凤英 马姜明 梁士楚 唐绍清 薛跃规   

  • 收稿日期:2013-09-29 修回日期:2013-11-18 出版日期:2014-04-20 发布日期:2014-04-20
  • 基金资助:
    广西师范大学项目“石蜡切片实验方法的改进及其在探究实验中的应用”;广西高等教育教学改革工程项目‘充分利用高校生物园构建生态学专业实践性教学新模式’

A Low-Toxicity and High-Effectiveness Preparation Method for Plant Paraffin Section

  • Received:2013-09-29 Revised:2013-11-18 Online:2014-04-20 Published:2014-04-20

摘要: 以乔木、灌木和藤本等不同类型植物叶及部分根茎为试验材料,对石蜡切片的主要技术环节进行了探究和改进。用天然有机溶剂松节油(oil of turpentine)代替二甲苯(xylene)为透明与脱蜡剂,以阿拉伯树胶(gum acacia powder)为贴片剂,采用番红整体染色与固绿分片染色相结合进行双重染色。试验结果表明,该方法可避免二甲苯的毒性危害,减少环境污染,缩短试验所需的时间,提高劳动效率和制片质量。

关键词: 染色体工程, 染色体工程, 远缘杂交, 遗传学性状, 单体附加系, 甜菜

Abstract: In this paper, exploration and improvement to the primary technical section was discussed base on the material of various leaves and roots from several plants including trees, bush and liane. During staining, Turpentine was used as transparent agent, and arabic gum was employed as adhesive. The safranin and fast green were binatively utilized in the staining process. A wealth of results showed that the ontamination from xylene could be avoided via our staining method, and the marked decline of pollution to environment was significant. Furthermore, the effectiveness and quality of the section were significantly improved with a shorter duration.