欢迎访问《农学学报》,

农学学报 ›› 2016, Vol. 6 ›› Issue (5): 60-64.doi: 10.11923/j.issn.2095-4050.cjas16010002

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

中国中北部青檀的AFLP分析

朱翠翠,张林,孙忠奎,王长宪   

  1. 山东农业大学林学院404室,泰安市泰山林业科学研究院,泰安时代园林科技开发有限公司,泰安市泰山林业科学研究院
  • 收稿日期:2016-01-04 修回日期:2016-01-25 接受日期:2016-02-18 出版日期:2016-06-01 发布日期:2016-06-01
  • 通讯作者: 朱翠翠 E-mail:zhucuicuitt@163.com
  • 基金资助:
    2014 年山东省农业良种工程项目“青檀种质创新利用与新品种培育”(鲁科字[2014]96 号)。

AFLP Analysis of Pteroceltis tatarinowii in the North of Central China

  • Received:2016-01-04 Revised:2016-01-25 Accepted:2016-02-18 Online:2016-06-01 Published:2016-06-01

摘要: 为揭示中国中北部青檀的遗传多样性,本研究从贵州、北京、甘肃、江苏、安徽、山东、河南7省12个种群收集了31 份青檀样品,对其进行AFLP分析。利用从26 对AFLP引物组合中筛选出的6对引物对样本DNA进行扩增,共产生223条谱带,其中211 条为多态性条带,比率达94.26%。结果显示中国中北部的青檀材料之间存在较高的遗传多样性,且AFLP 技术可以有效地对其进行分析。31 个样品的遗传相似系数在0.6413-0.9193之间,平均为0.7489,聚类分析结果显示样品首先按照种群聚在一起,但也有交叉。

关键词: 无人机机载激光雷达, 无人机机载激光雷达, 背包移动雷达, 刺槐地上生物量, 孤岛林场

Abstract: In order to study the genetic diversity of Pteroceltis tatarinowii in the north of central China, 31 samples were collected from 12 populations from 7 provinces (Guizhou, Beijing, Gansu, Jiangsu, Anhui, Shandong and Henan) and AFLP analysis was conducted. A total of 223 bands were generated from 6 primer combinations screened from 26 primer combinations and 211 of those bands showed polymorphism, the polymorphic ratio (PP) was up to 94.26%. The results showed that a high genetic diversity was existed among Pteroceltis tatarinowii samples from the north of central China and AFLP analysis was effective. The genetic similarity coefficient was in the range of 0.6413 to 0.9193, with an average value of 0.7489. And the results of cluster analysis showed that the samples were clustered together according to the population, but there were intersections between the populations.

中图分类号: