低温胁迫对蓖麻种子萌发过程中生理生化影响

doi:10.11923/j.issn.2095-4050.cjas16100008

农学学报 ›› 2017, Vol. 7 ›› Issue (3): 5-8.doi: 10.11923/j.issn.2095-4050.cjas16100008

所属专题：生物技术；油料作物

低温胁迫对蓖麻种子萌发过程中生理生化影响

白雪,李兴,刘鹏,郑根昌,李旭新,张春兰

内蒙古民族大学农学院,内蒙古民族大学农学院,内蒙古民族大学农学院,内蒙古民族大学农学院,内蒙古民族大学农学院,内蒙古民族大学生命科学学院

收稿日期:2016-10-10 修回日期:2016-12-15 接受日期:2016-12-23 出版日期:2017-03-27 发布日期:2017-03-27
通讯作者: 刘鹏 E-mail:1243940967@qq.com
基金资助:
内蒙古自治区高校蓖麻产业工程技术中心、内蒙古自治区蓖麻育种重点实验室科研项目“低温抑制蓖麻种子萌发的生理生化基础研究” (BMYJ2015-06)；市校合作项目“利用EMS优质抗逆蓖麻新品种的研究诱变选育”(SXYB2012050)。

Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean

张春兰

Received:2016-10-10 Revised:2016-12-15 Accepted:2016-12-23 Online:2017-03-27 Published:2017-03-27

摘要/Abstract

摘要： 旨在探索蓖麻种子在低温下萌发的调控机制，为选育优质抗逆蓖麻新品种做准备。笔者以3 个蓖麻品种为材料，采用完全随机设计，重复3 次，设置低温15℃、常温25℃(CK)，进行种子萌发试验，确定品种活力后，待胚根生长至2 cm时，进行取样测定相关生理指标。结果表明：低温显著阻碍蓖麻种子萌发。低温胁迫下可溶性糖含量和可溶性蛋白含量显著低于对照，而丙二醛含量显著高于对照；低温胁迫下供试蓖麻品种各酶活性表现差异较大，胚根SOD活性均极显著低于对照；除‘通蓖6 号’外，其余供试品种POD活性也极显著低于对照；CAT活性表现不显著，且不同温度条件下活性基本一致。通过试验得出‘通蓖5号’在低温胁迫下表现良好，可进行下一步基因测序。

关键词: 琯溪蜜柚, 琯溪蜜柚, 砂囊粒化, 采收期

Abstract: The purpose of the study is to explore the regulation mechanism of castor seed germination under low temperature, in order to select and breed new varieties with high quality and resistance. In this study, three castor cultivars were used as materials. A complete randomized design was adopted with 3 duplicates. The low temperature of 15℃ and normal temperature of 25℃ (CK) were set up and the seeds germination experiments were conducted. After determining the vitality of cultivars, the physiological index was determined by taking samples when the radicles were 2 cm long. The results indicated that seeds germination of castor was significantly hindered by low temperature stress. The sugar content and TSP were less than that of the control in radicles under low temperature, but the MDA content was significantly higher than that of the control under the low temperature. The performance of enzyme activity in castor was quite different under low temperature, SOD activity in radicles was less than that of the control under low temperature. Excepting‘Tongbi No.6’, other varieties’POD activity was significantly lower than that of the control; the CAT activity performance was not significant, and the activities were basically coincident under different temperature conditions. It concluded that‘Tongbi No.5’performed well under low temperature stress, and it could take gene sequencing for the next step.

白雪,李兴,刘鹏,郑根昌,李旭新,张春兰. 低温胁迫对蓖麻种子萌发过程中生理生化影响[J]. 农学学报, 2017, 7(3): 5-8.

张春兰. Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean[J]. Journal of Agriculture, 2017, 7(3): 5-8.

参考文献

[1].Koornneef M,Bentsink L,Hilhorst H.Seed dormancy and germination[J].Current Opinion in Plant Biology,2002,5(1) :33-36.
[2].Henderson I R,Dean C.Control of arabidopsis flowering: the chill before the bloom[J].Development,2004,131(16) :3829- 3838.
[3].Gray W M,Qstin A,Sandberg G,Romano C P,Estelle M.High temperature promotes auxin-mediated hypocotyl elongation in Arabidopsis[J].Proceedings of the National Academy of Sciences,1998,95(12): 7197-7202.
[4].Mazzella M A,Bertero D,Casal J J. Temperature-dependentinternode elongation in vegetative plants of Arabidopsis thaliana lacking phytochrome B and cryptochrome 1[J]. Planta,2000,210(3): 497-501.
[5].Penfield S.Temperature perception and signal transduction in plants[J].New Phytologist,2008,179(3) :615-628.
[6].魏乐,杜军华.高温和零上低温对小麦根系含糖量及细胞透性的影响[J].青海师范大学学报:自然科学版,1999,(1):36- 39.
[7].Baldwin BS, Cossar RD. Castor yield in response to planting date at four locations in the south-central United States. Industrial Crops and Products, 2009, 29:316-319.
[8].Ogunniyi DS. Castor oil: a vital industrial rawmaterial. Bioresource Technol, 2006, 97:1086–1091.
[9]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000,(7):184,164-167.
[10].马艳青, 戴雄泽. 2000.低温胁迫对辣椒抗寒性相关生理指标的影响[J].园艺学进展(第四辑),哈尔滨工程大学出版社, 374 ￥
[11].Gao Y,Qi X H,Yang J H,Zheng M F.The response mechanism of cold stress in higher plants[J].Northern Horticulture,2007(10) :58-61．
[12].Lu M H,Chen J F.2004.Genetic engineering of plant for cold tolerance[J]. Acta Botanica Boreali-Occidentalia Sinica.24(10):1953-1958.
[13].刘大永,王维香.过氧化氢对水稻幼苗中CAT和POD活性的影响[J].作物学报:1998,24(3):320-324.
[14].Susan W,Atul M,Vaidaya G B,et al.High specific activity of whole cells in an aqueousorganic two phasemem brane bioreactor [J].Enzyme and Technology,1998(22):575-577.
[15].潘晓云.膜脂过氧化作为扁桃品种抗寒性鉴定指标研究[J],生态学报,2002(11):1902-1911.
[16].简令成,吴素萱.植物抗寒性的细胞学研究-小麦越冬过程中细胞内物质的变化[J].植物报.1965,13: 1-16.
[17].Levitt J. Responses of Plant to Environmental Stress [M]. New York: Academic Press, 1980, 365-488.
[18].王爱国,邵从本,罗广华,等.大豆下胚轴线粒体的衰老与膜脂的过氧化作用[J].植物生理学报,1988,14(3):269.
[19].李明玉,曹辰兴,于喜艳.低温锻炼对冷胁迫下黄瓜幼苗保护性酶的影响[J].西北农业学报,2006,15(1):160-164.
[20].张自坤,刘世琦,王忠全,等.低温胁迫对不同砧木黄瓜嫁接苗生理生化指标的影响[J]..山东农业科学,2009,5:36-40.
[21].徐心诚.低温弱光对黄瓜幼苗过氧化物酶同工酶和丙二醛含量的影响[J].河南农业科学,2012,14(1):113-116.
[22].王燕,李雪平,季作梁.芒果冷害对两种自由基清除剂的影响[J].园艺学报,1995,22(3):235-239.
[23].朱其杰,高守云,蔡洙湖.等.黄瓜耐冷性鉴定及遗传规律的研究[M].见:李树德主编:中国主要菜抗病育种进展[M].北京:科学出版社,1995.457-462.

低温胁迫对蓖麻种子萌发过程中生理生化影响

Effects of Low Temperature Stress on Physiology and Biochemistry in Seed Germination of Castor Bean

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	詹婷, 胡承孝, 庄木来, 李潇彬, 雷靖, 聂敏, 杨涛, 赵小虎. 平和县琯溪蜜柚主产区果园营养状况及调控建议[J]. 农学学报, 2021, 11(10): 83-89.
[2]	胡彧娴. ‘琯溪蜜柚’中乙螨唑、啶虫脒的残留测定及套袋对残留的影响[J]. 农学学报, 2021, 11(1): 62-66.
[3]	孙恩虹,赵洁,韩旭,何永坤. 不同采收期江津青花椒气候品质变化[J]. 农学学报, 2019, 9(4): 88-91.
[4]	郭春景,王建忠,李广,林秋君. 乐果与氧乐果在露地芹菜中的残留动态及安全性评价[J]. 农学学报, 2018, 8(5): 6-9.