氯化镉对不同倍性泥鳅鳍细胞系的毒性效应

doi:10.11923/j.issn.2095-4050.cjas16110016

农学学报 ›› 2017, Vol. 7 ›› Issue (9): 52-57.doi: 10.11923/j.issn.2095-4050.cjas16110016

所属专题：生物技术

氯化镉对不同倍性泥鳅鳍细胞系的毒性效应

吴迪,李霞,秦艳杰,白丽雯,李状状

大连海洋大学水产与生命学院,大连海洋大学,大连海洋大学,大连海洋大学,大连海洋大学，辽宁省海洋生物资源恢复与生境修复重点实验室

收稿日期:2016-11-11 修回日期:2017-03-06 接受日期:2017-03-25 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 李霞 E-mail:qinyanjie
基金资助:
国家自然科学基金项目“泥鳅杂交三倍体染色体组稳定性及其表观遗传机制解析”(31272650)。

Cytotoxicity of Cadmium Chloride to Loach Fin Diploid and Triploid Cell Lines in Vitro

吴迪,,, and 李状状

Received:2016-11-11 Revised:2017-03-06 Accepted:2017-03-25 Online:2017-09-15 Published:2017-09-15

摘要/Abstract

摘要： 旨在研究不同浓度氯化镉对60~80 代二倍体和三倍体泥鳅鳍细胞系（DIMF和TRMF）的毒性效应，比较不同倍性细胞系间的敏感性差异。采用MTT比色法测定半致死浓度，采用酶活力测定法、微核试验和实时定量PCR法测定了氯化镉对2 种细胞系的细胞毒性和遗传毒性。结果表明：氯化镉处理下DIMF、TRMF细胞系半致死浓度分别为(24.0±0.7)、(27.3±1.3) μmol/L。2 种细胞系的SOD、GSH-Px活性随着氯化镉浓度的增加，呈现先升高后降低的趋势，而GST活性则表现为逐渐降低。随着氯化镉浓度的增加微核率先升高后降低，DIMF、TRMF最大微核率分别为(7.33±0.33)‰、(8.33±0.67)‰。经氯化镉处理的细胞金属硫蛋白(MT)表达量显著增加，30 μmol/L 浓度组表达量最大，分别为对照组的55.6 和57.6 倍。研究表明，氯化镉对泥鳅二倍体和三倍体细胞系具有细胞毒性和遗传毒性，且二倍体细胞系较三倍体细胞系对氯化镉更为敏感。

关键词: 渗透胁迫, 渗透胁迫, 甲基磺酸乙酯(EMS), 离体诱变, 大花萱草, 突变体率

Abstract: [Objective] In this study, 60-80 generation of loach fin diploid cell line (DIMF) and triploid cell line (TRMF) were used to assess toxicity of cadmium chloride in vitro, and the toxicity characteristics between the different ploidies were compared. [Method]Semi-lethal concentrations was determined with MTT method; Cytotoxicity and genotoxicity were studied through enzyme activity, micronuclei test, and Real-time quantitative PCR method. [Result]The semi-lethal concentrations of DIMF and TRMF were 24.0±0.7μmol/L and 27.3±1.3μmol/L, respectively. The results of enzymology showed the activities of SOD and GSH-Px were raised firstly, and then decreased. The activity of GST was decreased progressively with the increase of cadmium chloride concentration. Micronucleus rates were increased firstly and then decreased with the increase of cadmium chloride concentration, and the maximum rates of micronucleus were 7.33±0.33‰、8.33±0.67‰ respectively. The relative expression level of MT were significantly increased when the DIMF and TRMF were exposed by cadmium chloride. The maximum expression levels(55.6 and 57.6 fold of those in control) occurred when cadmium chloride concentration was 30μmol/L. [Conclusion]All results indicated that cytotoxicity and genotoxicity could be detected in DIMF and TRMF subjected to cadmium chloride, and DIMF was more sensitive to cadmium chloride than TRMF.

中图分类号:

Q256

吴迪,李霞,秦艳杰,白丽雯,李状状. 氯化镉对不同倍性泥鳅鳍细胞系的毒性效应[J]. 农学学报, 2017, 7(9): 52-57.

吴迪,,, and 李状状. Cytotoxicity of Cadmium Chloride to Loach Fin Diploid and Triploid Cell Lines in Vitro[J]. Journal of Agriculture, 2017, 7(9): 52-57.

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氯化镉对不同倍性泥鳅鳍细胞系的毒性效应

Cytotoxicity of Cadmium Chloride to Loach Fin Diploid and Triploid Cell Lines in Vitro

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