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农学学报

农学学报 ›› 2021, Vol. 11 ›› Issue (9): 7-10.doi: 10.11923/j.issn.2095-4050.cjas20191000235

• 农艺科学/生理生化 • 上一篇    下一篇

山药零余子EMS诱变处理的研究

南怀林(), 王耀琴, 刘建平, 王小兵, 张峰   

  1. 山西省农业科学院经济作物研究所,山西汾阳 032200
  • 收稿日期:2019-10-26 修回日期:2019-12-29 出版日期:2021-09-20 发布日期:2021-09-30
  • 作者简介:南怀林,男,1973年出生,山西稷山人,副研究员,本科,主要从事山药育种与栽培技术研究工作。通信地址：032200 山西省汾阳市小南关门外省农科院经济作物研究所,E-mail： nanhl517@163.com。
  • 基金资助:
    山西省农科院院生物育种工程“山药新品种选育”(17yzgc056)

EMS Mutagenesis of Chinese Yam Tuber

Nan Huailin(), Wang Yaoqin, Liu Jianping, Wang Xiaobing, Zhang Feng   

  1. Institute of Economic Crops, Shanxi Academy of Agricultural Sciences, Fenyang, Shanxi 032200
  • Received:2019-10-26 Revised:2019-12-29 Online:2021-09-20 Published:2021-09-30

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摘要：

旨在确定EMS诱变山药零余子的最佳处理浓度和时间。以‘晋山药1号’的零余子为材料,采用不同浓度EMS溶液和浸泡时间对零余子进行诱变处理,对处理后的零余子出苗率、成苗率、变异率进行比较。结果表明：当EMS溶液浓度小于0.5%,山药零余子的浸泡时间小于18 h,出苗率、成苗率、变异率随着浸泡时间延长趋势不变;浸泡时间大于18 h而小于36 h时,出苗率随着浸泡时间的延长呈现上升趋势,而变异率的趋势不变。EMS溶液浓度大于0.5%小于0.9%,且浸泡时间小于24 h,出苗率随着浸泡时间延长呈现下降趋势,变异率随着浸泡时间延长趋势上升。EMS溶液浓度等于0.9%,浸泡时间等于24 h,出苗率约为50%。EMS溶液浓度大于0.9%,浸泡时间大于30 h,变异率随着浸泡时间延长呈现下降趋势。综上,EMS诱变山药零余子的最佳处理浓度为0.9%、浸泡时间24~30 h。

关键词: 晋山药1号零余子, 甲基磺酸乙酯(EMS), 诱变, 浸泡时间, 出苗率, 成苗率, 变异率

Abstract:

The aim is to determine the optimal concentration and time of EMS mutation for Chinese yam tuber. Taking the tuber of ‘Jinshan 1’ as material, tuber mutagenesis was carried out by using different concentrations of EMS solution and soaking time. The emergence rate, seedling rate and variation rate of tuber were compared. The results showed that when the concentration of EMS solution was lower than 0.5% and the soaking time was shorter than 18 h, the emergence rate, seedling rate and variation rate remained unchanged with the prolongation of soaking time; when the soaking time was between 18 h and 36 h, the emergence rate increased with the prolongation of soaking time, but the variation rate remained unchanged. When the concentration of EMS solution was 0.5%-0.9% and the soaking time was less than 24 h, the emergence rate decreased and the variation rate increased with the prolongation of soaking time. When the concentration of EMS solution was 0.9% and soaking time was 24 h, the emergence rate was about 50%. When the concentration of EMS solution was more than 0.9% and the soaking time was longer than 30 h, the variation rate decreased with the prolongation of soaking time. Taken together, the optimal EMS solution is 0.9% and the best soaking time is 24-30 h for Chinese yam tuber.

Key words: Tuber of ‘Jin Shan Yao No.1’, EMS, Mutagenesis, Soaking Time, Emergence Rate, Seedling Rate, Variation Rate

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