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农学学报 ›› 2019, Vol. 9 ›› Issue (2): 72-77.doi: 10.11923/j.issn.2095-4050.cjas18030014

• 畜牧 兽医 水产 • 上一篇    下一篇

血浆种类和温度对褶纹冠蚌钩介幼虫非寄生变态发育及早期稚蚌生长的影响

马学艳, 闻海波, 金 武, 华 丹, 徐 跑, 顾若波   

  1. 中国水产科学研究院淡水渔业研究中心
  • 收稿日期:2018-03-15 修回日期:2018-06-13 接受日期:2018-06-22 出版日期:2019-02-13 发布日期:2019-02-13
  • 通讯作者: 顾若波 E-mail:maxy
  • 基金资助:
    江苏省自然科学基金面上项目“紫黑翼蚌钩介幼虫对寄主鱼特异性选择的分子机制研究”(BK20161145);中国水产科学研究院淡水渔业 研究中心基本科研业务费“紫黑翼蚌苗种规模化繁育关键技术研究”(2017GBFM03)。

Plasma Type and Temperature: Effects on the Non-parasitic Metamorphosis of Glochidia and Growth of Early Juvenile Mussel of Cristaria plicata

  • Received:2018-03-15 Revised:2018-06-13 Accepted:2018-06-22 Online:2019-02-13 Published:2019-02-13

摘要: 为完善褶纹冠蚌钩介幼虫体外培养技术和理论,采用体外培养技术研究了血清、温度对钩介幼虫的变态发育影响,并对早期稚蚌的成活和生长进行了评估。结果表明:鳙鱼血浆培养的钩介幼虫变态率极显著高于马血清、牛血清组(P<0.01);18℃培养的钩介幼虫变态率均显著高于30、24℃(P<0.05);褶纹冠蚌钩介幼虫生物学零度为9.34℃,有效积温为102.84℃·d;早期稚蚌养殖一个月后,鳙鱼血浆培养的稚蚌成活率最高(P<0.05),鳙鱼血浆组稚蚌壳长显著高于牛血清组、马血清组组(P<0.05);稚蚌在3 个养殖温度下,温度越低,成活率越高,且具有显著性差异(P<0.05)。综上所述,以鳙鱼血浆为营养源、18℃条件下培养的褶纹冠蚌钩介幼虫变态率最高,稚蚌养殖成活率高。

关键词: 金龟子, 金龟子, 趋性, 触角电位, 嗅觉相关蛋白

Abstract: To improve the in vitro culture of Cristaria plicata glochidia, the in vitro cultivation technique was adopted to study the effects of blood serum and temperature on metamorphosis of glochidia, and the survive and growth of juvenile mussel at earlier stage were evaluated. The results showed that the metamorphosis rate of glochidia in bighead plasma was significantly higher than that of horse serum and bovine serum group (P< 0.01); the metamorphosis rate of glochidia cultured at 18℃ was significantly higher than that at 30℃ and 24℃ (P<0.05), the biological zero point of Cristaria plicata glochidia was 9.34℃ and the effective accumulated temperature was 102.84℃· d. After one month cultivation of juvenile mussel at earlier stage, the survival rate of juvenile mussel cultured in bighead plasma was the highest (P<0.05), and the shell length of juvenile mussel in the bighead plasma group was significantly higher than that of bovine serum group and horse serum group (P< 0.05). Under the three different temperature for cultivation, the lower the temperature, the higher the survival rate, and the difference was significant (P<0.05). Taken together, Cristaria plicata larva cultivated with bighead plasma under 18℃ has the highest metamorphosis rate, and the mussel larva has the highest survival rate.