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农学学报

农学学报 ›› 2021, Vol. 11 ›› Issue (6): 36-41.doi: 10.11923/j.issn.2095-4050.cjas2020-0087

所属专题： 生物技术

• 植物保护 • 上一篇    下一篇

腐烂茎线虫对当归细胞结构和生理特性的影响

漆永红1(), 张新瑞1, 曹素芳2, 李敏权1, 李青青1, 李雪萍1, 蒋晶晶1, 李继平1()   

  1. 1甘肃省农业科学院植物保护研究所,兰州 730070
    2甘肃省农业科学院林果花卉研究所,兰州 730070
  • 收稿日期:2020-06-12 修回日期:2020-07-07 出版日期:2021-06-20 发布日期:2021-06-28
  • 通讯作者: 李继平 E-mail:qiyonghong920@gsagr.ac.cn;lijiping@gsagr.ac.cn
  • 作者简介:漆永红,男,1978年出生,甘肃秦安人,副研究员,博士,主要从事经济作物病害研究。通信地址：730070 甘肃省兰州市安宁区农科院新村1号 甘肃省农业科学院植物保护研究所,Tel：0931-7614844,E-mail： qiyonghong920@gsagr.ac.cn。
  • 基金资助:
    国家重点研发计划“特色经济作物化肥农药减施技术集成研究与示范”(2018YFD0201100);甘肃省农业科学院科研条件建设及成果转化项目“黄芩根腐病害关键防控技术研究”(2020GAAS25);甘肃省重点人才项目“党参根腐病综合防控技术集成、示范推广与人才培养”(2019RCXM072);嘉峪关市科技计划“嘉峪关市设施蔬菜根腐病综合治理技术研究与应用”(18-20)

Ditylenchus destructor: Effects on the Cell Structure and the Physiological Characteristics of Angelica sinensis

Qi Yonghong1(), Zhang Xinrui1, Cao Sufang2, Li Minquan1, Li Qingqing1, Li Xueping1, Jiang Jingjing1, Li Jiping1()   

  1. 1Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
    2Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2020-06-12 Revised:2020-07-07 Online:2021-06-20 Published:2021-06-28
  • Contact: Li Jiping E-mail:qiyonghong920@gsagr.ac.cn;lijiping@gsagr.ac.cn

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摘要：

为了明确当归受到腐烂茎线虫(Ditylenchus destructor)侵染后其根部细胞结构和叶部生理特性的变化,本试验以‘岷归1号’和腐烂茎线虫为材料,采用田间自然发病条件测定了腐烂茎线虫侵染后当归根部细胞结构,以及叶部生理特性的变化规律。徒手切片光学显微镜观察结果表明,腐烂茎线虫侵染后,根部发病部位细胞受到严重破坏和断裂。当归病叶片叶绿素含量降低,达到1.18 mg/g,电解质渗漏电导率升高,为76.19 ws/cm。当归病叶的净光合速率、气孔导度和蒸腾速率均降低,胞间CO2浓度升高;当归病叶光饱和点、最大净光合速率、暗呼吸速率和CO2饱和点均降低,而光补偿点和CO2补偿点均升高。同时当归病叶营养元素氮、磷、钾、锌、锰、铁的含量降低,分别为2.41、5.71、36.07、33.53、112.37、1318.03 g/kg,而铜、钙、镁的含量增加,分别达到8.07、538.13、33.13 g/kg。本研究为阐明当归麻口病的发病机制提供了理论依据。

关键词: 当归, 腐烂茎线虫, 细胞结构, 光和CO2响应, 生理特性, 营养元素含量

Abstract:

To determine the changes of root cell structure and leaf physiological characteristics of Angelica sinensis after infected with Ditylenchus destructor, ‘Mingui 1’ and D. destructor were used in this study. The cell structure of roots and the physiological characteristics of leaves were tested in filed by natural diseased condition. Microscopic observation showed that the cell structure of the diseased roots were seriously damaged and broken after infected with D. destructor. The chlorophyll content in diseased leaves decreased to 1.18 mg/g, while the electrolyte leakage conductivity increased to 76.19 ws/cm. The net photosynthetic rate, the stomatal conductance and transpiration rate decreased, whereas the concentration of intercellular CO2 increased. Moreover, compared with the control, the light saturation point, the maximum net photosynthetic rate, the dark respiration rate and the CO2 saturation point decreased, while the light compensation point and the CO2 compensation point increased. The content of nitrogen, phosphorus, potassium and some nutrient elements including Zn, Mn, Fe decreased to 2.41, 5.71, 36.07, 33.53, 112.37 and 1318.03 g/kg, respectively, while the content of Cu, Ca, Mg increased to 8.07, 538.13 and 33.13 g/kg, respectively. The results could provide a theoretical basis for elucidating the pathological mechanism of A. sinensis disease caused by D. destructor.

Key words: Angelica sinensis, Ditylenchus destructor, Cellular Structure, Light and CO2 Response, Physiological Characteristics, Nutrient Element Content

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