耿马甘蔗白叶病植原体昆虫介体调查与检测

doi:10.11923/j.issn.2095-4050.cjas2020-0137

农学学报 ›› 2022, Vol. 12 ›› Issue (5): 6-9.doi: 10.11923/j.issn.2095-4050.cjas2020-0137

所属专题：生物技术；植物保护

耿马甘蔗白叶病植原体昆虫介体调查与检测

李文凤¹(), 王晓燕¹, 仓晓燕¹, 张荣跃¹, 单红丽¹, 卢文洁²(), 王长秘¹, 尹炯¹, 黄应昆¹()

¹云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远 661699
²云南省农业科学院农生物技术与种质资源研究所,昆明 650205

收稿日期:2020-07-17 修回日期:2021-08-23 出版日期:2022-05-20 发布日期:2022-06-06
通讯作者: 卢文洁,黄应昆 E-mail:ynlwf@163.com;luwenjie1976@163.com;huangyk64@163.com
作者简介:李文凤,女,1964年出生,云南石屏人,研究员,学士,主要从事甘蔗病虫害研究。通信地址：661699 云南省开远市灵泉东路363号,Tel：0873-7227004,E-mail： ynlwf@163.com。
基金资助:
国家基金项目“甘蔗白叶病植原体的传播媒介及其传毒机理研究”(31760504);财政部和农业农村部国家现代农业产业技术体系专项资金“甘蔗真菌性病害防控岗位科学家”(CARS-170303);“云岭产业技术领军人才”培养项目“甘蔗有害生物防控”(2018LJRC56);云南省创新人才培养对象项目(202005AD160012)

Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma

LI Wenfeng¹(), WANG Xiaoyan¹, CANG Xiaoyan¹, ZHANG Rongyue¹, SHAN Hongli¹, LU Wenjie²(), WANG Changmi¹, YIN Jiong¹, HUANG Yingkun¹()

¹Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/ Yunnan Province Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, Yunnan, China
²Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China

Received:2020-07-17 Revised:2021-08-23 Online:2022-05-20 Published:2022-06-06
Contact: LU Wenjie,HUANG Yingkun E-mail:ynlwf@163.com;luwenjie1976@163.com;huangyk64@163.com

摘要/Abstract

摘要：

为探明云南蔗区甘蔗白叶病植原体的昆虫介体及优势种群,丰富甘蔗植原体病害相关理论和技术基础,制定适用于甘蔗白叶病的综合防控措施,2019年采用寻集法和扫网法对甘蔗白叶病发病最为严重的云南省临沧市耿马蔗区进行甘蔗白叶病植原体昆虫介体调查和巢式PCR检测分析。调查检测结果显示,采集到的大青叶蝉(Tettigoniella viridis)和条纹平冠沫蝉(Clovia conifer)2种昆虫介体均被检测为阳性,是甘蔗白叶病植原体的自然携带者,说明2种叶蝉可能为甘蔗白叶病植原体潜在昆虫介体;大青叶蝉若虫呈强阳性,初步确定为优势种群。鉴于目前云南蔗区甘蔗白叶病植原体传播方式主要是带毒蔗种和叶蝉类昆虫介体,建议建立甘蔗无病健康种苗繁育基地,及时杀灭蔗园中叶蝉类昆虫介体,从源头上控制甘蔗白叶病的扩散蔓延,降低田间自然传播速度。

关键词: 耿马, 甘蔗白叶病, 昆虫介体, 植原体, 巢式PCR

Abstract:

To investigate the insect vector and dominant population of sugarcane white leaf (SCWL) phytoplasma in Yunnan can enrich the theoretical and technical basis of sugarcane phytoplasma disease control and provide reference for formulating comprehensive control measures for SCWL. The insect vectors of SCWL phytoplasma were investigated using visual search and sweep netting methods and detected by nested PCR in Gengma of Lincang City, Yunnan Province in 2019, where the occurrence of SCWL was the most serious. The investigation and detection results showed that two collected insect vectors Tettigoniella viridis and Clovia conifer were tested positive. They were natural carriers of SCWL phytoplasma, indicating that these two leafhopper species might be the potential insect vectors of SCWL phytoplasma. Meanwhile, the nymph of T. viridis was tested strongly positive and preliminarily identified as the dominant population. In view of the fact that the main transmission ways of SCWL phytoplasma currently are SCWL-infected seedcane and leafhopper insect vector, it is suggested to establish a sugarcane breeding base of disease-free and healthy seedcane and kill leafhopper insect vector in sugarcane planting area in time, so as to control the spread of SCWL from the source and reduce the natural transmission speed in field.

Key words: Gengma, sugarcane white leaf disease, insect vector, phytoplasma, nested PCR

中图分类号:

S432.1

李文凤, 王晓燕, 仓晓燕, 张荣跃, 单红丽, 卢文洁, 王长秘, 尹炯, 黄应昆. 耿马甘蔗白叶病植原体昆虫介体调查与检测[J]. 农学学报, 2022, 12(5): 6-9.

LI Wenfeng, WANG Xiaoyan, CANG Xiaoyan, ZHANG Rongyue, SHAN Hongli, LU Wenjie, WANG Changmi, YIN Jiong, HUANG Yingkun. Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma[J]. Journal of Agriculture, 2022, 12(5): 6-9.

图/表 2

参考文献 27

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样品编号	昆虫介体种类及虫态	甘蔗白叶病巢式PCR检测结果
1	条纹平冠沫蝉成虫	–
2	条纹平冠沫蝉成虫	+
3	大青叶蝉若虫	+
4	大青叶蝉成虫	–
5	条纹平冠沫蝉若虫	+
6	条纹平冠沫蝉成虫	–
7	条纹平冠沫蝉成虫	–
8	条纹平冠沫蝉成虫	+
9	大青叶蝉成虫	+
10	大青叶蝉若虫	++
11	大青叶蝉成虫	–
12	条纹平冠沫蝉若虫	–
13	条纹平冠沫蝉成虫	–
14	大青叶蝉成虫	–
PC	阳性对照	++
NC	阴性对照	–
CK	空白对照	–

样品编号	昆虫介体种类及虫态	甘蔗白叶病巢式PCR检测结果
1	条纹平冠沫蝉成虫	–
2	条纹平冠沫蝉成虫	+
3	大青叶蝉若虫	+
4	大青叶蝉成虫	–
5	条纹平冠沫蝉若虫	+
6	条纹平冠沫蝉成虫	–
7	条纹平冠沫蝉成虫	–
8	条纹平冠沫蝉成虫	+
9	大青叶蝉成虫	+
10	大青叶蝉若虫	++
11	大青叶蝉成虫	–
12	条纹平冠沫蝉若虫	–
13	条纹平冠沫蝉成虫	–
14	大青叶蝉成虫	–
PC	阳性对照	++
NC	阴性对照	–
CK	空白对照	–

耿马甘蔗白叶病植原体昆虫介体调查与检测

Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma

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