尾水处理前后吉富罗非鱼几种免疫因子比较

doi:10.11923/j.issn.2095-4050.cjas2022-0045

农学学报 ›› 2025, Vol. 15 ›› Issue (2): 75-80.doi: 10.11923/j.issn.2095-4050.cjas2022-0045

尾水处理前后吉富罗非鱼几种免疫因子比较

尹统统¹(), 李全杰², 朱昊俊², 郑尧¹^,²()

¹ 南京农业大学无锡渔业学院，江苏无锡 214081
² 中国水产科学研究院淡水渔业研究中心/农业农村部稻渔综合种养生态重点实验室，江苏无锡 214081

收稿日期:2022-04-15 修回日期:2024-10-17 出版日期:2025-02-20 发布日期:2025-02-18
通讯作者:
郑尧，男，1986年出生，副研究员，博士，主要从事免疫学研究。通信地址：214081 江苏省无锡市滨湖区山水东路9号，Tel：0510-85550414，E-mail：zhengy@ffrc.cn。
作者简介:
尹统统，男，1997年出生，河北邯郸人，硕士，从事渔业环境保护。通信地址：214081 江苏省无锡市滨湖区山水东路9号，Tel：0510-85550414，E-mail：270340942@qq.com。
基金资助:
财政部和农业农村部国家现代农业产业技术体系资助“工程化养殖”(CARS-46)

Comparison of Several Immune Factors of Tilapia Before or After Waste Water Treatment

YIN Tongtong¹(), LI Quanjie², ZHU Haojun², ZHENG Yao¹^,²()

¹ Wuxi Fisheries College, Nanjing Agricultural University, Wuxi Jiangsu 214081
² Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs / Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081

Received:2022-04-15 Revised:2024-10-17 Online:2025-02-20 Published:2025-02-18

摘要/Abstract

摘要：

研究团队构建了“两坝三区”即“沉淀区-溢流坝-生态区-潜流坝-曝气区”耦合循环水生态养殖体系，并采用该尾水处理模式对全国九省市淡水养殖池塘进行了生态化改造。为探究改造是否具有提升鱼类免疫能力，本研究以吉富罗非鱼为研究对象，比较养殖尾水未处理与处理组血清中几种免疫因子含量的变化规律，并解决应用过程中成本核算这一产业关键问题。结果表明，处理过后的养殖尾水，并不会影响该批鱼特定生长率SGR和肝体指数HSI(P>0.05)，且对鱼体的生长指标无显著性影响(P>0.05)。血清免疫因子α1-AT和LYS含量，促炎因子(IFN-γ)和抗炎因子（IL-10和TGF-β）含量均显著增加(P<0.05)。研究发现，不同处理之间的罗非鱼生长差异与尾水浓度无关，养殖尾水处理后罗非鱼血清中几种免疫因子含量变化显著，可能通过激活巨噬细胞增强分泌型炎症因子，持续提高了其免疫能力。

关键词: 养殖尾水, 两坝三区模式, 成本核算, 炎症因子, 免疫因子

Abstract:

The team adopted the model of two dams and three zones to carry out ecological transformation of ponds in the Yangtze River Delta. In order to explore whether the transformation can improve the immune ability of fish, this study compared the changes of several immune factors in the serum of genetic improvement of farmed tilapia (GIFT) in the untreated and treated groups of waste water, and solved the key industrial problem of cost accounting in the application of the two dam and three zone model. Results showed that the treated aquaculture waste water would not affect the SGR and HSI of the tilapia, and had no significant effect on the growth index of the fish (P>0.05). The levels of immune factors α1-AT and LYS in GIFT were significantly increased, and the contents of pro-inflammatory factors (IFN-γ) and anti-inflammatory factors (IL-10 and TGF-β) were significantly increased (P<0.05). Significant changes in the levels of several immune factors have been observed in tilapia serum after treatment of aquaculture wastewater.

Key words: aquaculture waste water, two dams and three zones model, cost accounting, inflammatory cytokines, immune factor

尹统统, 李全杰, 朱昊俊, 郑尧. 尾水处理前后吉富罗非鱼几种免疫因子比较[J]. 农学学报, 2025, 15(2): 75-80.

YIN Tongtong, LI Quanjie, ZHU Haojun, ZHENG Yao. Comparison of Several Immune Factors of Tilapia Before or After Waste Water Treatment[J]. Journal of Agriculture, 2025, 15(2): 75-80.

图/表 7

分组	15 d SGR/(%/d)	15 d HSI/%	30 d SGR/(%/d)	30 d HSI/%
X组	4.71±0.84	3.50±0.55	4.06±0.27	2.42±0.40
Y组	5.29±0.69	3.43±0.22	3.45±0.45	2.32±0.15
Z组	6.61±0.90	4.23±0.27	4.14±0.37	1.88±0.21

表1. 不同浓度组对吉富罗非鱼生长指标的影响

图1. 处理前后对吉富罗非鱼血清α1-AT含量的影响不同小写字母表示显著性差异，下同

图2. 处理前后对吉富罗非鱼血清LYS含量的影响

图3. 处理前后对吉富罗非鱼血清IFN-γ含量的影响

图4. 处理前后对吉富罗非鱼血清IL-10含量的影响

图5. 处理前后对吉富罗非鱼血清TGF-β含量的影响

图6. 处理前后对吉富罗非鱼血清LPS含量的影响

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尾水处理前后吉富罗非鱼几种免疫因子比较

Comparison of Several Immune Factors of Tilapia Before or After Waste Water Treatment

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