PEDV乳酸菌工程菌株对小鼠的免疫效果研究

doi:10.11923/j.issn.2095-4050.cjas2023-0267

农学学报 ›› 2024, Vol. 14 ›› Issue (6): 67-71.doi: 10.11923/j.issn.2095-4050.cjas2023-0267

PEDV乳酸菌工程菌株对小鼠的免疫效果研究

安琦(), 牛彦波, 吴皓琼, 樊川, 曹亚彬, 原韬()

黑龙江省科学院微生物研究所，哈尔滨 150000

收稿日期:2023-12-05 修回日期:2024-01-10 出版日期:2024-06-19 发布日期:2024-06-19
通讯作者:
原韬，男，1979年出生，黑龙江哈尔滨人，副研究员，硕士，研究方向：农业微生物。通信地址：150010 哈尔滨市兆麟街68号黑龙江省科学院微生物研究所，Tel：0451-84614297，E-mail：talentee@qq.com。
作者简介:
安琦，1988年出生，黑龙江哈尔滨人，助理研究员，硕士，研究方向：农业微生物。通信地址：150010 哈尔滨市兆麟街68号黑龙江省科学院微生物研究所，E-mail：anqi2011stu@126.com。
基金资助:
黑龙江省科学院青年创新基金杰青项目“重组PEDV乳酸工程菌株构建研究”(CXJQ2021SW02)

Immune Effect of PEDV Lactobacillus Engineering Strain in Mice

AN Qi(), NIU Yanbo, WU Haoqiong, FAN Chuan, CAO Yabin, YUAN Tao()

Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, Heilongjiang, China

Received:2023-12-05 Revised:2024-01-10 Online:2024-06-19 Published:2024-06-19

摘要/Abstract

摘要：

本研究以引起猪流行性腹泻的猪流行性腹泻病毒(Porcine epidemic diarrhea virus，PEDV)为研究目标，利用含有PEDV S基因的植物乳杆菌活载体菌株LP1522-PEDS口服免疫小鼠，应用ELISA 试验检测免疫小鼠粪便中SIgA含量、血清中IgG、IL-4、IL-2和IFN-γ含量，评价乳酸菌重组菌株LP1522-PEDS对小鼠的免疫效果。结果表明，首次免疫后，商品灭活疫苗组和工程菌株免疫组粪便中的SIgA、血清中的IgG、IL-4、IL-2和IFN-γ含量较对照组显著升高(P<0.05)。在免疫42~56 d，灭活疫苗组和重组菌株组抗体水平均达到最大值，抗体和免疫因子水平依次为：商品灭活疫苗组>LP1522-PEDS组>空载组≈对照组。随后商品灭活疫苗组、LP1522-PEDS组抗体和免疫因子水平逐渐下降。免疫70 d，商品灭活疫苗组抗体水平降幅度相对较小，工程菌株免疫组降幅较大。结果表明，小鼠口服携带PEDV S基因的基因工程植物乳杆菌LP1522-PEDS后能够诱导机体产生PEDV免疫应答。

关键词: PEDV, 乳酸菌, 活载体疫苗, 小鼠免疫

Abstract:

Porcine epidemic diarrhea virus (PEDV), which causes porcine epidemic diarrhea, was used as the research target in this study. The live vector strain LP1522-PEDS of Lactobacillus plantarum containing PEDV S gene was used to immunize mice orally. The content of SIgA in feces and the contents of IgG, IL-4, IL-2 and IFN-γ in serum of immunized mice were detected by ELISA, and the immune effect of Lactobacillus recombinant strain LP1522-PEDS on mice was evaluated. The results showed that after the first immunization, the contents of SIgA in feces and IgG, IL-4, IL-2 and IFN-γ in serum of the commercial inactivated vaccine group and the engineered strain immunization group were significantly higher than those of the control group (P<0.05). The antibody levels of the inactivated vaccine group and the recombinant strain group reached the maximum after 42-56 days of immunization. The levels of antibodies and immune factors were as follows: commercial inactivated vaccine group > LP1522-PEDS group > empty vector group ≈ control group. Subsequently, the antibody levels in the inactivated vaccine group and LP1522-PEDS group gradually decreased. After 70 days of immunization, the antibody level of the commercial inactivated vaccine group decreased with small amplitude and the antibody level of the engineered strain immunization group decreased greatly. The results showed that the genetic engineering Lactobacillus plantarum LP1522-PEDS carrying PEDV S gene could induce PEDV immune response in mice via oral administration.

Key words: porcine epidemic diarrhea virus, lactic acid bacteria, vector vaccine, mouse immunization

安琦, 牛彦波, 吴皓琼, 樊川, 曹亚彬, 原韬. PEDV乳酸菌工程菌株对小鼠的免疫效果研究[J]. 农学学报, 2024, 14(6): 67-71.

AN Qi, NIU Yanbo, WU Haoqiong, FAN Chuan, CAO Yabin, YUAN Tao. Immune Effect of PEDV Lactobacillus Engineering Strain in Mice[J]. Journal of Agriculture, 2024, 14(6): 67-71.

图/表 5

图1. 小鼠血清中IgG抗体水平检测结果

图2. 小鼠免疫血清中IL-4含量 ng/L

图3. 小鼠免疫血清中IL-2含量 ng/L

图4. 小鼠免疫血清中IFN-γ含量

图5. 小鼠粪便中SIgA抗体水平检测

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PEDV乳酸菌工程菌株对小鼠的免疫效果研究

Immune Effect of PEDV Lactobacillus Engineering Strain in Mice

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