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农学学报 ›› 2011, Vol. 1 ›› Issue (2): 5-10.

所属专题: 小麦

• 农艺科学 生理生化 • 上一篇    下一篇

小麦抗蚜品种的遗传多样性SSR标记分析

仇松英 张松令 史忠良 谢福来 高炜 史晓芳   

  • 收稿日期:2011-03-21 修回日期:2011-03-29 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    山西省科技攻关项目“小麦外源抗逆基因的导入与利用研究”;山西省科技攻关项目“小麦抗麦红吸浆虫新品种选育及利用研究”

Genetic Diversity Analysis of Wheat Aphids Resistant Wheat Varieties by Simple Sequence Repeat (SSR) Markers

  • Received:2011-03-21 Revised:2011-03-29 Online:2011-04-25 Published:2011-04-25

摘要:

分析小麦抗蚜品种(系)的遗传多样性,为进一步培育和推广抗蚜品种提供依据。在田间对956份小麦品种(系)损失率鉴定结果的基础上,取47份年度间鉴定抗性结果较为一致的材料,按损失率大小,由小到大排列起来,利用筛选的18对多态性SSR标记检测了参试材料的遗传多态性。结果表明,18对SSR标记在47份不同抗性品种中检测到99个等位基因,能够将所有品种区分开来,每对引物可以检测到1~10个等位基因,平均为5.21个。47个小麦品种间的相对遗传距离在0.33~0.94之间,平均为0.65。高抗品种间相对遗传距离平均为0.59(0.39~0.77);中抗品种间的相对遗传距离平均为0.62(0.39~0.83);感虫品种间的相对遗传距离平均为0.62(0.37~0.83);高感品种间的相对遗传距离平均为0.64(0.37~0.84)。SSR标记聚类分析在相对遗传距离为0.67处将供试材料分为7大类群。选育和推广抗虫品种时尽可能选择聚类图中亲缘关系较远的材料。抗虫品种‘临远95-5322’单独聚为一类,同其余品种具有较远的亲缘关系,可作为新的抗源用于抗虫育种。

关键词: 贮藏, 贮藏

Abstract:

In order to gain knowledge of genetic diversity of wheat varieties/lines resistance to wheat aphids, on the basis of field insect plot appraisal, 47 wheat varieties/lines with different degrees of resistance to wheat aphids were studied by 18 SSR markers to explore genetic diversity of wheat aphids. 18 SSR markers were screened and a total of 99 allelic variants were detected in 47 wheat varieties ranged from 1 to 10 alleles per marker with an average of 5.21. The mean of alleles of high resistance insect (HR) wheat varieties were 3.83, of resistance insect (R) wheat varieties were 3.88, of sense insect (S) wheat varieties were 4.06 and of high sense insect (HS) wheat varieties were 3.56. The PIC (Polymorphism information contents, PIC) for each polymorphic primer varied from 0 to 0.91 with an average of 0.64. PIC of the HR wheat varieties were an average of 0.60 (0-0.97), of the R wheat varieties were an average of 0.64 (0-0.99), of the S wheat varieties were an average of 0.64 (0.14-0.85) and of the HS wheat varieties were an average of 0.67 (0-0.81). The mean genetic distance (GD) between 47 varieties was 0.65 varied from 0.33 to 0.94. The GD between HR variety was 0.59 varied from 0.39 to 0.77; The GD between R variety was 0.62 varied from 0.39 to 0.83. The GD between S varieties was 0.62, varied from 0.37 to 0.83. The GD between HS variety was 0.64, varied from 0.37 to 0.84. Based on 0.67 of GD, 47 varieties were clustered into seven groups. Resistant insect wheat variety ‘Linyuan 95-5322’ was a groups by oneself. It was explained that there was a further genetic relationship between ‘Linyuan 95-5322’ and other variety, and ‘Linyuan 95-5322’ could be used to resistant insect parent.

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