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农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (9): 51-55.

所属专题： 生物技术

• 农业工程 农业机械 生物技术 食品科学 • 上一篇    下一篇

不同酶解法水解豆粕蛋白的比较研究

宋永康 黄薇 林虬 姚清华 林香信   

  • 收稿日期:2012-05-07 修回日期:2012-07-06 出版日期:2012-09-20 发布日期:2012-09-20
  • 基金资助:

    利用生物技术进行饲料原料预处理的研究及应用

Comparative study of soybean protein hydrolysates prepared in different ways

  • Received:2012-05-07 Revised:2012-07-06 Online:2012-09-20 Published:2012-09-20

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摘要：

研究不同的酶解方式对豆粕蛋白酶解过程及产物的影响。采用Alcalase蛋白酶、Protex.7L蛋白酶对豆粕蛋白进行单酶、双酶同步和双酶分步水解，以水解度、蛋白质利用率、多肽得率、寡肽得率为指标对酶解过程进行分析。结果表明采用先加Alcalase蛋白酶后加Protex.7L蛋白酶组合水解豆粕蛋白的效果最佳，最终水解液中水解度可达23.5%，蛋白质利用率、多肽得率、寡肽得率分别为68.7%、51.0%、21.4%。双酶分步水解的效果优于单酶水解和双酶同步水解；酶的加入顺序对豆粕的酶解过程有一定的影响。

关键词: 丹江口水库, 丹江口水库, 消落带, 土壤样品, 总氮含量

Abstract:

In the present study, Alcalase protease and Protex.7L protease were utilized to hydrolyze soybean protein in different ways. i.e., single-enzyme hydrolysis as well as one-step and stepwise double-enzyme hydrolysis. After degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield were measured to comparatively study soybean protein hydrolysate in different hydrolysis way. The results showed that stepwise double-enzyme hydrolysis was superior to single-enzyme hydrolysis and one-step double-enzyme hydrolysis, and sequential hydrolysis with Alcalase protease followed by Protex.7L protease was the best way. Under this way, the degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield reached 23.5%, 68.7%, 51.0%, 21.4%.

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