温湿度对草莓炭疽菌侵染草莓叶片的影响

doi:10.11923/j.issn.2095-4050.cjas19030005

农学学报 ›› 2020, Vol. 10 ›› Issue (1): 22-26.doi: 10.11923/j.issn.2095-4050.cjas19030005

所属专题：园艺；农业气象

温湿度对草莓炭疽菌侵染草莓叶片的影响

赵玳琳, 何海永, 杨学辉()

贵州省农业科学院植物保护研究所,贵阳 550006

收稿日期:2019-03-04 修回日期:2019-07-22 出版日期:2020-01-22 发布日期:2020-01-22
通讯作者: 杨学辉 E-mail:yxuehui66@163.com
作者简介:赵玳琳,女,1987出生,贵州安顺人,助理研究员,硕士,主要从事植物真菌病害的生物防治研究工作。通信地址：550006 贵州省贵阳市花溪区金竹镇贵州省农业科学院,E-mail：dlsczhao@sina.com
基金资助:
贵州省农业科学院专项“贵州木霉菌资源收集、鉴定与多样性分析”(黔农科院院专项2016029号);贵州省科研机构服务企业行动计划项目“贵州五种特色果蔬病虫害绿色防控技术体系服务企业行动计划”(黔科合服企20154012)

Strawberry Leaves Infected by Colletotrichum fragariae: Effect of Temperature and Humidity

Zhao Dailin, He Haiyong, Yang Xuehui()

Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China

Received:2019-03-04 Revised:2019-07-22 Online:2020-01-22 Published:2020-01-22
Contact: Xuehui Yang E-mail:yxuehui66@163.com

摘要/Abstract

摘要：

为探索草莓炭疽病潜伏侵染条件提供理论依据。本研究在离体条件下通过调控室内温湿度对草莓炭疽病的发病情况及侵染特性差异性进行研究。结果表明：温湿度组合处理25℃/85%、20℃/80%、15℃/80%、10℃/80%、15℃/75%、10℃/75%在接种7天后均能发病,处理10℃/70%接种后28天才轻微发病。侵染特性差异性研究表明,处理25℃/85%,接种6 h,25%的分生孢子开始萌发;接种12 h,附着孢开始形成,形成率为16%,接种48 h,叶片开始出现零星病斑;接种7天,叶片组织细孢大量腐烂崩解,病指为39.07。处理10℃/70%,接种1天,2.88%分生孢子开始萌发;直至接种24天,分生孢子萌发率达到100%,附着孢开始形成,形成率为8.83%;接种28天,叶片开始出现零星病斑,病指仅为1.85;接种42天,叶片组织大面积腐烂,形成典型的炭疽病斑。得出处理10℃/70%接近该病害发病的底线温湿度条件,此条件下的潜伏期能够达到28天。

关键词: 草莓, 草莓炭疽病, 温湿度, 附着孢, 潜伏侵染, 病情指数

Abstract:

To provide a basis for exploring the condition of latent infection of strawberry leaves infected by Colletotrichum fragariae, we studied the morbidities and the differences of infection characteristics of strawberry leaves infected by Colletotrichum fragariae in vitro by regulating the indoor temperature and humidity. The results showed that: the combinations of temperature and humidity of 25℃/85%, 20℃/80%, 15℃/80%, 10℃/80%, 15℃/75% and 10℃/75% all could cause disease after being inoculated 7 d, however the treatment of 10℃/70% could cause slight disease after being inoculated 28 d. The differences of infection characteristics showed that: 25% of conidia germinated after being inoculated 6 h of treatment of 25℃/85%; after 12 h, appressorium began to form and the formation rate was 16%; after 48 h, sporadic disease spots began to appear on the leaves; after 7 d, a mass of tissue cells of leaves began to decompose, and the disease index was 39.07; in the treatments of 10℃/70%, after 1 d, 2.88% of conidia germinated; the germination rate of the conidia was 100% and 8.83% appressorium began to form after being inoculated 24 d; after 28 d, sporadic disease spots began to be appear on the leaves and the disease index was 1.85; after 42 d, a mass of tissue cells of leaves began to decompose and typical anthracnose disease spots were found on the leaves. In conclusion, 10℃/70% is close to the baseline of temperature and humidity condition of strawberry leaves infected by Colletotrichum fragariae, and the incubation period can reach 28 d under the condition.

Key words: Strawberry, Colletotrichum fragariae, Temperature and Humidity, Appressoria, Latent Infection, Disease Index

中图分类号:

赵玳琳, 何海永, 杨学辉. 温湿度对草莓炭疽菌侵染草莓叶片的影响[J]. 农学学报, 2020, 10(1): 22-26.

Zhao Dailin, He Haiyong, Yang Xuehui. Strawberry Leaves Infected by Colletotrichum fragariae: Effect of Temperature and Humidity[J]. Journal of Agriculture, 2020, 10(1): 22-26.

图/表 4

参考文献 25

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调查时间/d	处理
调查时间/d	25℃/85%	20℃/80%	15℃/80%	10℃/80%	15℃/75%	10℃/75%	10℃/70%
7	39.07a	33.33a	7.41b	3.70b	3.70b	1.85b	0.00b
9	47.25a	37.04a	9.26b	5.56b	7.41b	3.70b	0.00b
11	65.67a	55.78a	15.07b	10.32b	13.47b	8.25b	0.00b
24	100.00a	100.00a	100.00a	100.00a	92.59a	77.78a	0.00b
28	100.0a	100.00a	100.00a	100.00a	100.00a	88.89a	1.85b

调查时间/d	处理
调查时间/d	25℃/85%	20℃/80%	15℃/80%	10℃/80%	15℃/75%	10℃/75%	10℃/70%
7	39.07a	33.33a	7.41b	3.70b	3.70b	1.85b	0.00b
9	47.25a	37.04a	9.26b	5.56b	7.41b	3.70b	0.00b
11	65.67a	55.78a	15.07b	10.32b	13.47b	8.25b	0.00b
24	100.00a	100.00a	100.00a	100.00a	92.59a	77.78a	0.00b
28	100.0a	100.00a	100.00a	100.00a	100.00a	88.89a	1.85b

接种时间/h	分生孢子萌发率/%	附着孢形成率/%
3	0.00d	0.00c
6	25.00c	0.00c
9	90.00b	0.00c
12	100.0a	16.00b
24	100.0a	70.00a

接种时间/h	分生孢子萌发率/%	附着孢形成率/%
3	0.00d	0.00c
6	25.00c	0.00c
9	90.00b	0.00c
12	100.0a	16.00b
24	100.0a	70.00a

接种时间/d	分生孢子萌发率/%	附着孢形成率/%
1	2.88f	0.00d
4	20.00e	0.00d
8	30.5d	0.00d
12	48.78c	0.00d
16	90.41b	0.00d
20	98.50a	0.00d
24	100.00a	8.83c
28	100.00a	73.33b
32	100.00a	89.33a

温湿度对草莓炭疽菌侵染草莓叶片的影响

Strawberry Leaves Infected by Colletotrichum fragariae: Effect of Temperature and Humidity

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