关键词: 绿盲蝽, 绿盲蝽, 精氨酸激酶, 枣园, 葡萄园, 荧光定量

Abstract:

Antagonism of Trichoderma aureoviride strain T1010 against Fusarium solani strain F5 was assessed and its effect was analyzed. Antagonistic action of strain T1010 against strain F5 was determined by dual culture assay, meanwhile interactions between strain T1010 and F5 was observed through microscopy. Strain T1010 controlled strain F5 and reduced pepper root rot was investigated in pot tests and solar-greenhouse trails. The results showed that: strain T1010 grew quickly and covered whole dish in 2.99 days, meanwhile strain F5 needed 11.41 days. Strain T1010 competed against F5 in same dish, and the inhibition rate was 83.28%. Tropic growth of strain T1010 hyphae dissolved F5’ cells, and T1010’ coverage rate was 95.67%. In the greenhouse, there was little of population of F5, meanwhile the population of T1010 was 6.285×105 cfu/g of soil mixed with T1010+F5. There was little population of T1010 and F5 into pepper stem and root. However, the population of F5 was 6.385×104 cfu/g of soil without T1010 treatment. There were a lot of F5 hyphae in the stem and root of pepper. The powder formulation of T1010 was applied into soil in pot trail, compared with the control, the rate of germination increased, the infected plant reduced, pepper height and root length increased by 1.81 times, 4.38 times. The powder formulation of T1010 was applied by broadcast in the solar-greenhouse, pepper lateral root was increased. Pepper disease infecting index decreased up to 2.61, production increased 24.61% compared with carbendazim treatment. Strain T1010 possesses multiple mechanisms of antifungal activity. The study proposed that 150 kg/hm2 T1010 preparation applied was the best for biocontrol pepper root rot in solar-greenhouse and should be popularized.