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农学学报 ›› 2017, Vol. 7 ›› Issue (9): 42-47.doi: 10.11923/j.issn.2095-4050.cjas17060014

所属专题: 园艺

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

不同浓度NaCl对‘达米娜’葡萄影响的主成分分析

谭 伟,李晓梅,董志刚,谭 敏,茹慧玲,唐晓萍   

  1. 山西省农业科学院果树研究所,山西省农业科学院果树研究所,山西省农业科学院果树研究所,山西省农业科学院果树研究所,山西省农业科学院果树研究所,山西省农业科学院果树研究所
  • 收稿日期:2017-06-19 修回日期:2017-06-30 接受日期:2017-07-17 出版日期:2017-09-15 发布日期:2017-09-15
  • 通讯作者: 唐晓萍 E-mail:tanweisxgss
  • 基金资助:
    山西省农业科学院农业科技创新研究课题“盐胁迫下外源钙调节葡萄活性氧代谢的机理”(ZDSYS1505);山西省重点研发计划重点项目 “葡萄产业提质增效关键技术研发与示范”(201603D21105)。

Principal Component Analysis of the Effect of Different NaCl Concentrations on ‘Tamina’Grape Seedlings

  • Received:2017-06-19 Revised:2017-06-30 Accepted:2017-07-17 Online:2017-09-15 Published:2017-09-15

摘要: 为筛选NaCl 胁迫对葡萄影响的主要指标,建立评价葡萄抗盐性体系提供理论依据,以沙培1 年生‘达米娜’葡萄自根苗为试验材料,研究6 个浓度NaCl胁迫10 天后,葡萄根系和叶片相对电导率、叶片超氧阴离子产生速率、H2O2含量、抗氧化酶(SOD、CAT、POD、APX)活性、植株新梢长度和总生物量的变化。结果表明:与对照相比,随着NaCl 浓度的升高,根系和叶片相对电导率呈逐渐上升趋势,叶片超氧阴离子产生速率和H2O2含量增加,叶片中SOD和CAT活性呈先升高后降低的趋势,APX活性呈现“降低—升高—降低”的趋势,而POD活性呈下降的趋势;高浓度NaCl 胁迫下,新梢长度和植株总生物量降低,叶片超氧阴离子产生速率和H2O2含量增加可能与较低的抗氧化酶活性有关。通过主成分分析得到,新梢长度、叶片CAT活性、H2O2含量和APX活性可作为不同浓度NaCl胁迫对葡萄影响的评价指标。

关键词: PEG, PEG, 谷子, 种子活力

Abstract: The paper aims to select the main indexes of the effects of NaCl stress on grapevine and provide theory basis for evaluation the salt resistance system of grapevines. With the annual of grapes ‘Tamina’ grown on the sand as the experimental material, six concentration of NaCl treatments were conducted, then the relative conductivity of grape root and leaf, the oxygen anion production rate, H2O2 content, antioxidant enzyme (SOD, CAT, POD, APX) activity of leaves, shoot length and total biomass of plants were analyzed. Results showed that the root and leaf relative conductivity increased gradually with the increase of NaCl concentration compared with the control, the super oxygen anion production rate and H2O2 content increased, SOD and CAT activity in the leaves presented a trend of rise-down, APX activity presented a trend of down-rise-down, while POD activity presented a downward trend; under high concentrations of NaCl treatment, the length of shoot and total biomass of the plants were reduced, higher oxygen anion production rate and H2O2 content in grape leaves might be related with the lower antioxidant enzyme activity. The principal component analysis indicated that the shoot length, CAT activity, H2O2 content and APX activity of leaves could be used to evaluate the effects of different concentration of NaCl stress on grapevine.

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