欢迎访问《农学学报》，
农学学报

农学学报 ›› 2011, Vol. 1 ›› Issue (2): 49-53.

• 畜牧 兽医 水产 • 上一篇    下一篇

一株亚硝酸盐降解菌的分离鉴定

周冬仁 沈锦玉 潘晓艺 叶雪平 徐洋 尹文林   

  • 收稿日期:2011-03-22 修回日期:2011-04-08 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    水质改良有益微生物制剂的研究与开发

Isolation and Identification of Nitrite-oxidizing Bacteria

  • Received:2011-03-22 Revised:2011-04-08 Online:2011-04-25 Published:2011-04-25

PDF (PC)

2313

摘要：

为寻找对亚硝酸盐具有高效降解能力、稳定和安全的优良菌株，采用亚硝酸盐氧化细菌(Nitrite-oxidizing bacteria, NOB)富集、分离技术从湖州某水域淤泥中分离得到一株纯培养菌株N3。提取细菌的总DNA，利用细菌16S rDNA特异性引物进行多聚酶链反应(PCR)扩增；扩增产物经琼脂糖凝胶电泳和Sanger末端终止法测序分析，用NCBI-Blast软件将测序结果在Genbank等数据库中进行同源性检索。结果表明，N3的DNA扩增产物片断大小为1441 bp，测序结果经检索证实N3与标准菌株Cellulosimicrobium cellulans (AY665978.1) 16S rDNA保守性片段有100%的同源性，可以判定所分离得到的N3菌株为纤维单胞菌属的纤维化纤维单胞菌(Cellulosimicrobium cellulans)。

关键词: 优势, 优势

Abstract:

Objective to study the isolation and characterization of the nitrite-oxidizing bacteria and identification by 16S rDNA. Methods one pure culture N3, which was enriched and isolated based on the procedures for nitrite oxidizer from sludge of a lake in Huzhou. Total DNA of pure culture was extracted and specific primers for the 16S rDNA of nitrite oxidizer were used for polymerase chain reaction (PCR). The amplified product was analyzed by agarose gel e1ectrophoresis and Sanger sequencing. Homology analysis was made by searching in Genbank with NCBI-Blast. Results the amplified fragment was 1441 bp for N3. 100% of N3 nucleotides were identica1 with the conserved fragment of Cellulosimicrobium cellulans (AY665978.1). Conclusion N3 phy1ogenetica1ly belonged to Cellulosimicrobium cellulans.

中图分类号: