Abstract: To ascertain the change of soil physical and chemical properties in the bamboo forest in Lin’an, an investigation was made on the status of fertilization and soil samples were gathered in the main bambooplanting area in Lin’an. The results showed the grave acidification of the topsoil (0-20 cm), with the lowest pH value of 3.2 and a trend of declining in pH value with the depth of the soil, and the accumulation of organic matter and available phosphorus in the topsoil, with the contents of 57.6 g/kg and 765.0 mg/kg respectively. By analysis, the long-term over-application of chemical fertilizers, especially nitrogen and phosphorus fertilizers, has caused the imbalance of soil nutrients in the bamboo forest, leading to a fall in soil pH and the grave acidification of the topsoil (0-20 cm), with the lowest value of soil pH of 3.2, well below the suitable soil pH value of 5.5-7.0 for the growth of the bamboo, along with a significant increase in available phosphorus and potassium, with the highest contents of 693.0 and 507.0 mg/kg respectively. In the conventional practice of bamboo planting, a combination of over-application of chemical fertilizers, acid matter released by a large amount of organic materials covering the topsoil and organic acid excreted by the bamboo plant contributed to the significant acidification of the bamboo forest.