Abstract: To research the effect of different light quality and the light intensity treatment on the growth of Anoectochilus roxburghii tissue culture seedling, the LED light source was used as an artificial light source for A.roxburghii in Zhangzhou to subculture. The results showed that red light treatment was to the benefit of the growth of A.roxburghii height, a inhibitioning effect on growth of tissue culture seedling, accumulation of biomass, chlorophyll, Fv/Fm and Fv/Fo of A.roxburghii under the blue light treatment. Among them, the shoot high of A.roxburghii was up to 6.26 cm under 70 μmol/(m2·s) red light, but the chlorophyll a, chlorophyll b, total content of chlorophyll, Fv/Fm and Fv/Fo were only 1.19 mg/g, 0.55 mg/g, 1.73 mg/g, 0.572 and 1.34; it was not obvious that the effect of green light for the indicators of A.roxburghii, and no significant difference between different intensity; a promoting effect on accumulation of biomass and chlorophyll and Fv/Fm and Fv/Fo of A.roxburghii under the blue light treatment, but certain inhibitory effect on plant height, among them, the chlorophyll a, chlorophyll b, total content of chlorophyll, fresh weight, dry weight, the ratio of dry weight to fresh weight, Fv/Fm and Fv/Fo were up to 1.47 g, 0.19 g, 0.130, 1.83 mg/g, 0.71 mg/g, 2.54 mg/g, 0.801 and 4.02, but the shoot high of A.roxburghii was only 4.56 cm. The red light was advantageous to the growth of aerial parts of plants, the green light had little impact on plant photosynthesis, and certain effect on dwarfed and strong seedling of tissue culture seedling under the blue light.