Abstract: [The study aims to improve per unit yield and nitrogen use efficiency in southwest hilly and mountain area winter paddy fields represented by Chongqing and achieve the target of high yield and high efficiency in regional rice production and an environmental friendly production process. The author selected‘Yuxiang 203’ as material, took the method of field trial and studied the effects of fertilizer application amount and methods on accumulation and distribution of biological yield, nitrogen use efficiency and rice yield in heading to mature stage. The results showed that, rice biological yield, nitrogen accumulation quantity and grain yield increased with the increase of nitrogen application rate. Use nitrogen fertilizer as heading fertilizer was conducive to accumulation and rational distribution of biological yield, when the nitrogen rate was 10 kg/666.7 m2, base N fertilizer and jointing N fertilizer proportion was 5:5, the output percent and transportation percent of stemsheath store matter were higher, which were 56.59% and 48.59% , respectively. Use nitrogen fertilizer as heading fertilizer was conducive to increase agronomy utilization efficiency and apparent utilization efficiency, the nitrogen application ration varied with nitrogen application. Use nitrogen fertilizer as heading fertilizer was conducive to increase plain number of grains per ear, seed setting rate and thousand grain weight, and achieved large panicle and high production. Giving consideration to both grain yield and nitrogen utilization, efficient nitrogen application strategy of hybrid rice in Chongqing winter paddy field was as follows: the nitrogen rate was 10 kg/666.7 m2, the proportion of the base N fertilizer and jointing N fertilizer was 6:4 or 5:5, actual grain yield was 9910.68-9940.62 kg/hm2, the average grain yield was 9925.65 kg/hm2.