Abstract: In order to investigate a rapid propagation cultivation technique system of Pyrus calleryana Decne, Pyrus calleryana Decne‘Capital’was used as experimental materials. Different sterilization time, different basic culture media, proliferation culture and tube seedlings rooting culture, and the choosing of cultivation medium were studied. The results showed that 14 min was the best length of 5 different times in the sterilization process, although the rate of survival was lower than that of 16 min, but the explants sprouted quickly and grew normally. MS was the basic culture medium. Adding 0.5 mg/LTDZ, 0.2 mg/L NAA, 0.05 mg/L GGR6, 6.0 g/L AGAR powder, 30 g/L sucrose, 2.0 g/L activated carbon to MS was the optimal proliferation medium formula. Adding 0.5 mg/L TDZ, 1.5 mg/L ABT1#, 20 g/L sucrose, 4.5 g/L AGAR powder, 2.0 g/L activated carbon to MS was the optimal rooting medium formula. Transplanting survival rate was the highest, and the plants had the best growth performance, robust growing, and dark green leaf color when peat and perlite (a ratio of 2:1) were used as the cultivation medium.