Abstract: In order to find out the grain medium that appropriate mycelium growth of Pleurotus for provides evidence of Pleurotus mycelium cultivate and extract, preparation planting material. The study carrying out in experiment on microbiology using randomized block design. The tested materials of this experiment ware Pleurotus nebrodensis No.1 and Pleurotus nebrodensis No.801. The affection on the growth of Pleurotus nebrodensis from seven kinds of grain medium(shelling and no shelling) were explorated through comparing mycelium germination time, color, density, uniformity, the average growth rate and growth index. The results showed that whether shelling or not, Both Pleurotus nebrodensis No.1 and Pleurotus nebrodensis No.801 can grow in the 7 kinds of grain medium, but the growth situation was different. Shelling sorghum culture medium was the most suitable for Pleurotus nebrodensis mycelium growth, The followed medium was no shelling wheat、buckwheat and sorghum. Through comparing, except buckwheat, shelling culture medium was the most suitable for Pleurotus nebrodensis No.1 mycelium growth, its growth index was very high. Except proso millet, the no shelling grain medium was the least suitable for Pleurotus nebrodensis No.801 mycelium growth, its growth index was very high. Taking everything into account, shelling or not, sorghum、wheat and barley culture medium were comparatively suitable for Pleurotus nebrodensis mycelium growth.