Phenotype Analysis of Pineapple Hybrid Line Obtained by Mixed-pollen Cross and Its Paternal Origin Analysis

doi:10.11923/j.issn.2095-4050.cjas17070006

Abstract

Abstract: This work was to investigate the paternal origin of pineapple hybrid line H obtained by mixed-pollen cross. In this work, the phenotypes were investigated by comparing the growth characteristics and quality properties of H with its female parent as well as the two pollen supporters. Additionally, the genetic similarity and SNP gene source of H with its female and the two pollen supporters were calculated based on euclidean distance analysis and SLAF-seq. The obtained results suggested that the phenotypic parameters such as the phenology, plant size, as well as the quality and color aspects of fruit were quite alike to those of Yellow Mauritius, which was one of the pollen supporters, and some physical-chemical characteristics of H excelled. The aroma components of H were similar to those of female parent Smooth Cayenne. The size of fruit of H was lower than that of Smooth Cayenne and Comte de.Paris, while higher than Yellow Mauritius. The analysis of euclidean distance of phenotype parameters suggested that the genetic similarity between H and Yellow Mauritius was higher than that between H and Comte de.Paris. SLAF-seq showed that the possibility of H''s paternal origin from Yellow Mauritius was greater than that of Comte de.Paris. H''s paternal origin was probably from Yellow Mauritius.

References

[1] VARELA M C, BRAS R, BARROS I R, et al. Opportunity for hybridization between two oak species in mixed stands as monitored by the timing and intensity of pollen production[J]. Forest Ecology and Management, 2008, 256: 1546-1551.
[2] 曾贞, 罗军武, 杨阳, 等. 混合花粉授粉茶树杂交F1代AFLP分析[J]. 湖南农业大学学报(自然科学版), 2008, 34(6): 719-723.ZENG Z, LUO J W,SYANG Y, et al. AFLP analyses of the F1 progenies of tea from a mixed-pollen cross[J]. Journal of Hunan Agricultural University (Natural sciences), 2008, 34(6): 719-723.
[3] 梁月荣, 田中淳一, 武田善行. 应用RAPD分子标记分析“晚绿”品种的杂交亲本[J]. 茶叶科学, 2000, 20(1): 22-26.LIANG Y R, TANAKA J, TAKEDA Y. Parentage analysis of tea clone“Okumidori”with RAPD method[J]. Journal of Tea Science, 2000, 20(1): 22-26.
[4] 王江民, 陈素梅, 滕年军, 等. 基于形态性状的菊属与亚菊属植物亲缘关系的研究[J]. 植物遗传资源学报, 2013, 14(6): 1031-1037.WANG J M, CHEN S M, TENG N J, et al. Phylogenetic relationship analysis of several species from Chrysanthemum and Ajania according multivariate morphological characteristics[J]. Journal of Plant Genetic Resources, 2013, 14(6): 1031-1037.
[5] 孔志新, 王亚, 孙明, 等. 地被菊品种及其近缘种的数量分类[J]. 东北林业大学学报,2013,41(3): 73-77, 81.KONG Z X, WANG Y, SUN M, et al. Numerical classification of ground-cover dendranthema and its relative germplasm[J]. Journal of Northeast Forestry University, 2013,41(3): 73-77, 81.
[6] 杨柳慧, 于晓南. 基于表型性状的芍药不同品种群亲缘关系分析[J]. 植物遗传资源学报, 2016, 17(2): 209-216.YANG L H,YU X N. Phylogenetic relationship analysis of different cultivars groups of herbaceous peony according morphological characteristics[J]. Journal of Plant Genetic Resources, 2016, 17(2): 209-216.
[7] 武耀廷, 张天真, 朱协飞, 等. 陆地棉遗传距离与杂种F1、F2产量及杂种优势的相关分析[J]. 中国农业科学, 2002, 35(1): 22-28.WU Y T, ZHANG T Z, ZHU X F, et al. Relationship between F1, F2 yield heterosis and genetic distance measured by molecular markers and parents performance in cotton[J]. Seientia Agricultura Sinica, 2002, 35(1): 22-28.
[8] 倪先林, 张涛, 蒋开锋, 等. 杂交稻特殊配合力与杂种优势、亲本间遗传距离的相关性[J]. 遗传, 2009, 31(8): 849-854.NI X L, ZHANG T, JIANG K F, et al. Correlations between specific combining ability, heterosis and genetic distance in hybrid rice[J]. Hereditas, 2009, 31(8): 849-854.
[9] 刘新龙, 蔡青, 吴才文, 等. 甘蔗品种资源的表型遗传多样性[J]. 生物多样性, 2010, 18(1): 37-43.LIU X L, CAI Q, WU C W, et al. Phenotypic diversity of sugarcane variety germplasm[J]. Biodiversity Science, 2010, 18(1): 37-43.
[10] 王小萍, 唐晓波, 王迎春, 等. 52 份茶树资源生化组分的表型多样性分析[J]. 茶叶科学, 2012, 32(2): 129-134.WANG X P, TANG X B, WANG Y C, et al. Phenotypic diversity analysis of biochemical composition in 52 tea germplasms[J]. Journal of Tea Science, 2012, 32(2): 129-134.
[11] 苏文瑾, 赵宁, 雷剑, 等. 基于SLAF-seq 技术的甘薯SNP 位点开发[J]. 中国农业科学, 2016, 49(1): 27-34.SU W J, ZHAO N, LEI J, et al. SNP Sites developed by specific length amplification fragment sequencing (SLAF-seq) in sweetpotato[J]. Scientia Agricultura Sinica, 2016, 49(1): 27-34.
[12] 陈士强, 秦树文, 黄泽峰, 等. 基于SLAF-seq技术开发长穗偃麦草染色体特异分子标记[J]. 作物学报, 2013, 39(4): 727-734.CHEN S Q, QIN S W, HUANG Z F, et al. Development of specific molecular markers for thinopyrum elongatum chromosome using SLAF-seq technique[J]. Acta Agronomica Sinica, 2013, 39(4): 727-734.
[13] 彭强, 叶生鑫, 黄龙, 等. 运用SLAF-seq 技术构建水稻高密度遗传图谱[J]. 分子植物育种, 2016, 14 (8): 2127-2132.PENG Q, YE SHENG X, HUANG L, et al. Construction of a high-density genetic map in rice by using specific-length amplified fragment sequencing (SLAF-seq) technology[J]. Molecular Plant Breeding, 2016, 14 (8): 2127-2132.
[14] 仝月澳, 周厚基. 果树营养诊断法[M]. 北京: 农业出版社. 1982.
[15] LIU C H, LIU Y. Impacts of shading in field on micro-environmental factors around plants and quality of pineapple fruits[J]. Journal of Food, Agriculture Environment, 2012, 10(2): 741-745.
[16] 武耀廷, 张天真, 殷剑美. 利用分子标记和形态学性状检测的陆地棉栽培品种遗传多样性[J]. 遗传学报, 2001, 28(11):1040 -1050.WU Y T, ZHANG T Z, YIN J M. Genetic diversity detected by DNA markers and phenotypes in upland cotton[J]. Acta Genetica Sinica, 2001, 28(11):1040 -1050.
[17] 陈香玲, 苏伟强, 刘业强, 等. 36份菠萝种质的遗传多样性SCoT分析[J]. 西南农业学报, 2012, 25(2): 625-629.CHEN X L, SU W Q, LIU Y Q, et al. Analysis on genetic diversity of 36 pineapple collections by SCoT markers[J]. Southwest China Journal of Agricultural Sciences, 2012, 25(2): 625-629.
[18] 窦美安, 张惠云, 孙伟生, 等. 菠萝无刺卡因和巴厘杂交F1的苗期表型分析[J]. 热带作物学报, 2011, 32(8): 1431-1433.DOU M A, ZHANG H Y, SUN W S, et al. Phenotypes Analysis of the Pineapple F1 Seedlings from the Cross of Smooth Cayene×Comte de Paris[J]. Chinese Journal of Tropical Crops, 2011, 32(8): 1431-1433.
[19] KAEWTATHIP T, CHAROENREIN S. Changes in volatile aroma compounds of pineapple (Ananas comosus) during freezing and thawing[J]. International Journal of Food Science and Technology, 2012, 47: 985-990.
[20] 冯丽媛, 何祥凤, 王文和, 等. 利用SRAP分子标记技术对混合授粉后的百合F1代父本鉴定[J]. 沈阳农业大学学报, 2015, 46(2): 230-233.FENG L Y, HE X F, WANG W H, et al. Identification of paternal parent of the f1 progenies in lily genus from a mixed-pollen cross using SRAP[J]. Journal of Shenyang Agricultural University, 2015, 46(2): 230-233.
[21] 王斯琪, 唐诗哲, 孔德仓, 等. 利用SSR标记进行枣树子代苗父本鉴定[J]. 园艺学报, 2012, 39(11): 2133-2141.WANG S Q, TANG S Z, KONG D C, et al. Application of SSR markers for the identification of paternal parent for the seedlings of Chinese jujube[J]. Acta Horticulturae Sinica, 2012, 39(11): 2133-2141.
[22] 刘子记, 李静婷, 杨衍, 等. 辣椒杂交种SSR分子标记鉴定及表型比较分析[J]. 华北农学报, 2014, 29(1): 69 -72.LIU Z J, LI J T, YANG Y, et al. SSR molecular markers identification of pepper hybrid and comparative analysis with phenotypes[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(1): 69 -72.
[23] 赵欣欣, 张新全, 苗佳敏, 等. 多花黑麦草杂交种SSR分子标记鉴定及表型比较分析[J]. 农业生物技术学报, 2013, 21(7): 811-819.ZHAO X X, ZHANG X Q, MIAO J M, et al. Identification by SSR molecular markers and comparative analysis in morphological characterization in Lolium multiflorum hybrid progenies[J]. Journal of Agricultural Biotechnology, 2013, 21(7): 811-819.
[24] KAUSHIK N, KUMAR K, KUMAR S, et al. Genetic variability and divergence studies in seed traits and oil content of Jatropha (Jatropha curcas L.) accessions[J]. Biomass and Bioenergy, 2007, 31: 497-502.
[25] KLIMKO M, BORATYNSKA K, MONTSERRAT J M, et al. Morphological variation of Juniperus oxycedrus subsp. Oxycedrus (Cupressaceae) in the Mediterranean region[J]. Flora, 2007, 202: 133-147.
[26] GANOPOULOS I, MOYSIADIS T, XANTHOPOULOU A, et al. Diversity of morpho-physiological traits in worldwide sweet cherrycultivars of GeneBank collection using multivariate analysis[J]. Scientia Horticulturae, 2015, 197: 381-391.
[27] 范凯, 洪永聪, 丁兆堂, 等. 茶树‘黄山种’自然杂交后代遗传多样性分析[J]. 园艺学报, 2010, 37(8): 1357-1362.FAN K, HONG Y C, DING Z T, et al. Analysis of genetic diversity among natural hybrid progenies of Camellia sinensis‘Huangshanzhong’ [J]. Acta Horticulturae Sinica, 2010, 37(8): 1357-1362.