Abstract: Different ways of sterilization and medium formula were used for tissue culture and rapid propagation of Crytocoryne Affinis. The results showed that good effect obtained by using young germ as explant soaking in 75% ethanol and 0.05% mercuric chloride for 30s and 15mins respectively, and washed with aseptic water for 5 times. The appropriate medium for bud growth is MS+coconut juice 40 g..L-1+sucrose 10 g.L-1+ activated charcoal.1 g.L-1+agar powder 5 g.L-1 . Medium with MS+NAA0.1 mg.L-1+IBA0.2 mg.L-1+coconut juice 40 g.L-1+sucrose 20 g.L-1+ activated charcoal2 g.L-1+agar powder 4 g.L-1 is good for healthy seedling and roots development. Crytocoryne Affinis seedling by tissue culture and rapid propagation were transplanted, water growth acclimated and quantity produced smoothly.