Population Genetics of Chinese Mitten Crab Between the Breeding and Wild Populations

doi:10.11923/j.issn.2095-4050.cjas2020-0158

Abstract

Abstract:

Eriocheir sinensis, especially the Yangtze mitten crab, is an important aquaculture species in China. In recent years, serious germplasm mixture of this species has been detected and little is known about the reasons. In this study, population genetics among wild and breeding populations of the Chinese mitten crab was comparatively analyzed based on mitochondrial marker in order to explore the germplasm mixed level. The results show that the genetic diversity of wild crab in the Yangtze River is low, and over fishing could cause the decline of wild resources. No genetic difference among the wild and breeding populations is detected, which means serious germplasm mixture. This study clarifies the resource status of Chinese mitten crab in the Yangtze River, and can provide a scientific basis for the protection of this species.

Key words: Chinese mitten crab, the breeding and wild populations, mitochondrial gene, population genetics analysis, germplasm mixture

CLC Number:

S917.4

ZHOU Huaxing, DUAN Guoqing, JIANG He, LING Jun, HU Yuting. Population Genetics of Chinese Mitten Crab Between the Breeding and Wild Populations[J]. Journal of Agriculture, 2022, 12(6): 55-59.

Figures/Tables 6

References 38

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群体类型	样本采集地	缩写	样本数量/尾
养殖群体	宣州区	XZ	30
	当涂县	DT	40
	无为县	WW	30
	芜湖县	WH	40
野生群体	长江流域芜湖-马鞍山段	YS	30

群体类型	样本采集地	缩写	样本数量/尾
养殖群体	宣州区	XZ	30
	当涂县	DT	40
	无为县	WW	30
	芜湖县	WH	40
野生群体	长江流域芜湖-马鞍山段	YS	30

基因	引物名	引物序列	退火温度
COII	PXCOII-F	5’-CTTATCCTCCAGCCGACC-3’	55℃
COII	PXCOII-R	5’-TGGCATAAATAGTAATTCACAG-3’	55℃

基因	引物名	引物序列	退火温度
COII	PXCOII-F	5’-CTTATCCTCCAGCCGACC-3’	55℃
COII	PXCOII-R	5’-TGGCATAAATAGTAATTCACAG-3’	55℃

群体	单倍型数量	单倍型分布（个体数）	Hd	Pi
XZ	7	Hap1 (3), Hap2 (20), Hap3 (1), Hap4 (3), Hap6 (1), Hap8 (1), Hap12 (1)	0.5490	0.0034
DT	5	Hap1 (17), Hap2 (16), Hap4 (4), Hap9 (2), Hap7 (2)	0.6628	0.0011
WW	5	Hap1 (3), Hap2 (23), Hap5 (2), Hap7 (1), Hap10 (1)	0.4090	0.0006
WH	6	Hap1 (13), Hap2 (21), Hap3 (1), Hap4 (1), Hap7 (3), Hap10 (1)	0.6270	0.0010
YS	4	Hap1 (10), Hap2 (18), Hap8 (1), Hap11 (1)	0.5450	0.0008