Immune Effect of PEDV Lactobacillus Engineering Strain in Mice

doi:10.11923/j.issn.2095-4050.cjas2023-0267

Abstract

Abstract:

Porcine epidemic diarrhea virus (PEDV), which causes porcine epidemic diarrhea, was used as the research target in this study. The live vector strain LP1522-PEDS of Lactobacillus plantarum containing PEDV S gene was used to immunize mice orally. The content of SIgA in feces and the contents of IgG, IL-4, IL-2 and IFN-γ in serum of immunized mice were detected by ELISA, and the immune effect of Lactobacillus recombinant strain LP1522-PEDS on mice was evaluated. The results showed that after the first immunization, the contents of SIgA in feces and IgG, IL-4, IL-2 and IFN-γ in serum of the commercial inactivated vaccine group and the engineered strain immunization group were significantly higher than those of the control group (P<0.05). The antibody levels of the inactivated vaccine group and the recombinant strain group reached the maximum after 42-56 days of immunization. The levels of antibodies and immune factors were as follows: commercial inactivated vaccine group > LP1522-PEDS group > empty vector group ≈ control group. Subsequently, the antibody levels in the inactivated vaccine group and LP1522-PEDS group gradually decreased. After 70 days of immunization, the antibody level of the commercial inactivated vaccine group decreased with small amplitude and the antibody level of the engineered strain immunization group decreased greatly. The results showed that the genetic engineering Lactobacillus plantarum LP1522-PEDS carrying PEDV S gene could induce PEDV immune response in mice via oral administration.

Key words: porcine epidemic diarrhea virus, lactic acid bacteria, vector vaccine, mouse immunization

AN Qi, NIU Yanbo, WU Haoqiong, FAN Chuan, CAO Yabin, YUAN Tao. Immune Effect of PEDV Lactobacillus Engineering Strain in Mice[J]. Journal of Agriculture, 2024, 14(6): 67-71.

Figures/Tables 5

References 26

[1]	JUNG K, SAIF L J, WANG Q. Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control[J]. Virus research, 2020, 286(1):198045-198063.
[2]	CHEN P, WANG K, HOU Y, et al. Genetic evolution analysis and pathogenicity assessment of porcine epidemic diarrhea virus strains circulating in part of China during 2011-2017[J]. Infection, genetics and evolution, 2019, 69(1):153-165.
[3]	吴中杉. 规模化猪场流行性腹泻综合性防控措施的探讨[D]. 扬州: 扬州大学, 2022.
[4]	牛江婷, 张华, 伊淑帅, 等. 猪流行性腹泻病毒拮抗干扰素产生的分子机制研究进展[J]. 中国预防兽医学报, 2018, 40(1):87-90.
[5]	梅小强, 曾忠花, 徐函, 等. 猪流行性腹泻病毒及其卵黄抗体研究进展[J]. 畜牧与兽医, 2019, 51(7):127-131.
[6]	HOU Y, WANG Q. Emerging highly virulent porcine epidemic diarrhea virus: Molecular mechanisms of attenuation and rational design of live attenuated vaccines[J]. International journal of molecular sciences, 2019, 20(21):5478-5492.
[7]	徐瑞, 王改琴, 耿文静, 等. 乳酸菌素对母猪繁殖性能及哺乳仔猪生长性能的影响[J]. 饲料工业, 2023, 44(11):36-40.
[8]	何进, 徐思杨, 刘波, 等. 乳酸菌在农业和食品加工中的应用研究进展[J]. 微生物学杂志, 2022, 42(4):1-11.
[9]	宋翔, 侯玉凤, 陈晓雯. 1株具有免疫增强功能的乳酸菌筛选及其免疫效果评价[J]. 畜牧与兽医, 2022, 54(1):49-54.
[10]	刘晓琳, 张传健, 郭仕琦, 等. 猪流行性腹泻新型疫苗的研究进展[J]. 中国动物传染病学报,2021:1-10.
[11]	汪梦竹, 蒲飞洋, 赵泽阳, 等. 新型疫苗的研究进展[J]. 畜牧与兽医, 2022, 54(9):141-147.
[12]	李婉. 猪流行性腹泻病毒感染性克隆构建及其抗病毒药物和疫苗研究[D]. 武汉: 华中农业大学, 2022.
[13]	樊平, 颜邦斌, 王怀禹. 猪流行性腹泻病毒流行毒株S1蛋白的原核表达与鉴定[J]. 黑龙江畜牧兽医, 2019(17):93-96.
[14]	邓益超. 表达猪流行性腹泻病毒S1基因重组乳酸杆菌免疫原性的初步探究[D]. 成都: 四川农业大学, 2019.
[15]	GUO M R, YI S S, GUO Y B, et al. Construction of a recombinant Lactococcus lactis strain expressing a variant porcine epidemic diarrhea virus s1 gene and its immunogenicity analysis in mice[J]. Viral immunology, 2019, 32(3):144-150.
[16]	MA S T, WANG L, HUANG X W, et al. Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus[J]. Microbial cell factories, 2018, 17(1):20-32. doi: 10.1186/s12934-018-0861-7 pmid: 29426335
[17]	XIAO Y, ZHAO J X, ZHANG H, et al. Mining lactobacillus and bifidobacterium for organisms with long-term gut colonization potential[J]. Clinical Nutrition, 2020, 39(5):1315-1323. doi: S0261-5614(19)30225-0 pmid: 31174942
[18]	韩雪冰, 元香南, 方俊, 等. 乳酸菌维持动物肠道健康的研究进展[J]. 中国科学:生命科学, 2023, 53(4):464-479.
[19]	顾芳, 胡平, 蔡德敏, 等. 畜禽健康养殖中抗生素应用及其替代品研究进展[J]. 动物营养学报, 2023, 35(10):6247-6256. doi: 10.12418/CJAN2023.574
[20]	MAO Y, ZHANG X, XU Z. Identification of antibacterial substances of Lactobacillus plantarum DY-6 for bacteriostatic action[J]. Food science & nutrition, 2020, 8(6):2854-2863.
[21]	亓秀晔, 刘乃芝, 程福亮, 等. 乳酸菌用作口服疫苗表达载体的应用研究进展[J]. 中国酿造, 2019, 38(6):18-23. doi: 10.11882/j.issn.0254-5071.2019.06.004
[22]	陈妍卉, 刘军亭, 于潜, 等. 重组细菌活载体疫苗研究进展[J]. 中国预防兽医学报, 2022, 44(2):219-224.
[23]	VAN DIEP N, SUEYOSHI M, NORIMINE J, et al. Molecular characterization of US. like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013-2016 and PEDVs collected from recurrent outbreaks[J]. BMC veterinary research, 2018, 14(1):90-96.
[24]	何海健, 吴瑗, 王鲁彦, 等. PEDV分离株S1基因的重组杆状病毒真核表达中国畜牧兽医, 2019, 46(3):832-839.
[25]	WANG J L, HUANG L L, MOU C X, et al. Mucosal immune responses induced by oral administration recombinant Bacillus subtilis expressing the COE antigen of PEDV in newborn piglets[J]. Bioscience reports, 2019, 39(3):2018-2028.
[26]	JOSHI L R, OKDA F A, SINGREY A, et al. Passive immunity to porcine epidemic diarrhea virus following immunization of pregnant gilts with a recombinant of virus vector expressing the spike protein[J]. Archives of virology, 2018, 163(9):2327-2335.