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农学学报

农学学报 ›› 2011, Vol. 1 ›› Issue (2): 34-39.

所属专题： 生物技术

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

矮牵牛的查尔酮合酶(CHS)基因的生物信息学分析

汪结明 王凤玲 李瑞雪 魏万亮   

  • 收稿日期:2011-04-08 修回日期:2011-04-18 出版日期:2011-04-25 发布日期:2011-04-25
  • 基金资助:

    湖南省教育厅项目

Analysis on the Bioinformatics of Chalcone Synthetase Gene in Petunia hybrida Vilm.

  • Received:2011-04-08 Revised:2011-04-18 Online:2011-04-25 Published:2011-04-25

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摘要：

为研究CHS(chalcone synthase)基因间的同源性及其进化关系，以矮牵牛的CHS基因为研究对象，利用生物信息学软件及网站对其进行碱基分布、氨基酸组成和亲疏水性预测，并与其他10个物种的CHS基因序列进行多重比对、进化分析。结果表明：矮牵牛的CHS基因的单链mRNA序列含有1170个碱基对，蛋白质由389个氨基酸编码，相对分子质量为42580.02，其中亮氨酸(Leu)含量最高达10.80%；疏水值最大为2.038，最小为-2.208，无明显的亲水或疏水区域；多重比对发现11个物种同源性达到了80.55%。通过以上结果得出CHS基因处于稳定状态，编码的蛋白为亲水性蛋白，进化过程中是保守的，获得的保守区段的序列信息为新基因的克隆奠定了基础。

关键词: 保护, 保护

Abstract:

To study the homology and evolutionary relations among CHS (chalcone synthase) genes, CHS gene in Petunia hybrida, Vilm. was took as explants material, base distribution, amino acids composition, prediction of hydrophobicity or hydrophilicity, and multiple alignments with other 10 species were analyzed by the software and bioinformatics web site. The results showed that the mRNA single sequence contained 1170 bp base, the number of amino acids encoding protein was 389 and its relative molecular mass was 42580.02, Leucine achieved the highest content of 10.80%, hydrophobic maximum value was 2.038, hydrophobic minimum value was -2.208, and no obvious hydrophilic or hydrophobic area. The homology of multiple alignment of the amino acid sequence for CHS genes in 11 species achieved 80.55%. The results showed that the genes were in a stable state, encoded proteins were hydrophilic protein, and the evolutionary process was conservative. The conservative sequence information achieved in these CHS genes laid a foundation for other new CHS gene cloning.