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农学学报

农学学报 ›› 2012, Vol. 2 ›› Issue (3): 31-35.

所属专题： 生物技术

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

非洲菊的组织培养研究

杨尧 任雪 杜兴翠 赖齐贤 郭巧会 章四庆   

  • 收稿日期:2011-09-08 修回日期:2011-12-16 出版日期:2012-03-20 发布日期:2012-03-20

Tissue Culture of Gerbera jamesonii

  • Received:2011-09-08 Revised:2011-12-16 Online:2012-03-20 Published:2012-03-20

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摘要：

为提供非洲菊化学诱变的基础材料以及相应的组培操作技术，采用非洲菊品种‘琳达’的幼嫩花托为外植体进行组织培养研究，外植体最佳消毒时间为流水冲洗1 h，75%的酒精浸泡30 s，0.1%的HgCl2灭菌12 min；诱导产生愈伤组织的最适培养基为：MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+蔗糖3% (w/v)+琼脂粉7% (w/v)；增殖培养基配方MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖3% (w/v)+琼脂粉7% (w/v)为最适配方；生根壮苗培养基以1/2MS+NAA 0.1 mg/L+蔗糖2%+琼脂粉7%为最佳。实验显示以花托为外植体较为容易进行表面灭菌，诱导愈伤组织并分化丛生芽，从而快速建立离体快繁体系。

关键词: 水产品消费量, 水产品消费量, 人均GDP, 动态关系

Abstract:

To provide the basis of the materials for Gerbera’s chemical inducement; and the corresponding set of technique. Using gerbera varieties 'Linda' ’s young receptacle as explants to tissue culture. The results showed that: the best sterilization time on explants was 12 minutes, the callus introduction medium for in vitro culture was MS(Murashige and Skoog) + 6-BA 5.0mg/L +NAA0.5mg/L+ surge3% + agar7%, the multiplication medium was MS+ 6-BA 1.0mg/L +NAA0.1mg/L+ surge3% + agar7%, the rooting medium was 1/2MS + NAA0.1mg/L + surge2%+ agar7%.