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农学学报 ›› 2024, Vol. 14 ›› Issue (2): 42-53.doi: 10.11923/j.issn.2095-4050.cjas2023-0055

• 土壤肥料 资源环境 生态 • 上一篇    下一篇

内生细菌对中国特有植物青檀根际土壤肥力变化的影响

蒋银妹(), 覃勇荣(), 张燕桢, 黄秀连, 邓慧莲, 莫单玉, 蒙恩如   

  1. 河池学院化学与生物工程学院,广西宜州 546300
  • 收稿日期:2023-03-01 修回日期:2023-05-16 出版日期:2024-02-20 发布日期:2024-02-16
  • 通讯作者:
    覃勇荣,1963年出生,广西平南人,教授,硕士,研究方向:桂西北岩溶地区石漠化治理及生态恢复。通信地址:546300 广西河池市宜州区龙江路42号,Tel:0778-3141174,E-mail:
  • 作者简介:

    蒋银妹,女,2001年出生,广西全州人,学士,研究方向:喀斯特岩溶地区植物资源开发利用与生态环境保护。通信地址:541000 广西全州县中板桥村10号,Tel:0773-4651010,E-mail:

  • 基金资助:
    广西自然科学基金“广西不同植被恢复模式对石漠化地区生态恢复效果的比较研究”(桂科自0832273); 广西高校校地校企合作科技创新平台“桂西北地方资源保护与利用工程中心”(桂教科研[2012]9号); 河池学院高层次人才科研启动费项目“桂西北岩溶地区石漠化治理优化模式研究”(XJ2018GKQ016); 广西高校大学生创新创业训练计划项目“内生菌对中国特有植物任豆根际土壤肥力变化的影响”(202210605026)

Effects of Endophytic Bacteria on Rhizosphere Soil Fertility Changes of Chinese Endemic Plant Pteroceltis tatarinowii

JIANG Yinmei(), QIN Yongrong(), ZHANG Yanzhen, HUANG Xiulian, DENG Huilian, MO Danyu, MENG Enru   

  1. School of Chemistry and Bio-engineering, Hechi University, Yizhou 546300, Guangxi, China
  • Received:2023-03-01 Revised:2023-05-16 Online:2024-02-20 Published:2024-02-16

摘要:

为了探究内生菌对中国特有植物青檀根际土壤肥力变化的影响,选取桂西北常见乡土树种青檀以及石山造林先锋树种任豆为研究对象,采集自然植被样地青檀和人工林对照样地任豆的非根际土壤样品、根际土壤样品和根系样品为试验材料,利用常规的土壤理化分析、细菌培养及16S rRNA测序技术相结合的研究方法,依次分析研究树种非根际土壤细菌、根际土壤细菌及根内生细菌的群落结构特征,并在门和属两个不同水平,对根内生细菌与土壤理化因子的相关性进行分析,进一步说明内生细菌对青檀根际土壤肥力变化的影响。结果表明:(1)不同植被调查样地细菌种群数量具有明显差异,无论是非根际土壤细菌、根际土壤细菌,还是根内生细菌,任豆人工林对照样地明显高于青檀自然植被样地;(2)根内生细菌与根际土壤细菌及非根际土壤细菌彼此联系,相互作用,既有一定数量的共有种,又有各自的特有种,而且特有种的数量明显大于共有种;(3)在不同植被调查样地,菌群数量大小排序为:根际土壤细菌>非根际土壤细菌>根系内生菌;(4)青檀自然植被样地和任豆人工林对照样地,土壤细菌和根内生细菌的优势菌门组成相似,但相对丰度不同,其共有种的优势菌群均为芽孢杆菌属,该属的大部分细菌能在一定程度上改善植物的生长环境,增强植物的抗逆性和抗病能力。因此,青檀的根内生细菌与任豆及其他植物的根内生细菌一样,可以在一定程度上提高根际土壤肥力。

关键词: 中国特有植物, 青檀, 内生细菌, 根际土壤, 肥力变化

Abstract:

To investigate the effects of endophytes on rhizosphere soil fertility of the endemic Chinese plant Pteroceltis tatarinowii, the common native tree species P. tatarinowii in northwest Guangxi Zhuang Autonomous Region and the pioneer tree species of the stone mountain afforestation, Zenia insignis, were selected as research subjects. Non-rhizosphere soil samples, rhizosphere soil samples and root samples of P. tatarinowii from natural vegetation plots and Z. insignis from plantation control plots were collected as experimental materials, using the conventional methods of soil physical and chemical analysis, bacterial culture and 16S rRNA sequencing, the community structure characteristics of non-rhizosphere soil bacteria, rhizosphere soil bacteria and root endophytic bacteria of tree species were analyzed respectively. The correlation between root endophytic bacteria and soil physical and chemical factors was analyzed at two different levels of phyla and genera, which further explained the effect of endophytic bacteria on the changes of soil fertility of P. tatarinowii rhizosphere. The results showed that: (1) there were significant differences in bacterial populations among different vegetation plots, whether it was non-rhizosphere soil bacteria, rhizosphere soil bacteria or root endophytic bacteria, the contrast plots of Z. insignis plantation were obviously higher than that of the natural vegetation plots of P. tatarinowii; (2) root endophytic bacteria, rhizosphere soil bacteria and non-rhizosphere soil bacteria were related to each other and interacted with each other. There were not only certain number of common species, but also their own unique species, and the number of unique species was obviously larger than that of common species; (3) in different vegetation plots, the order of microflora was rhizosphere soil bacteria>non-rhizosphere soil bacteria>root endophyte; (4) the dominant phyla composition of soil bacteria and root endophytic bacteria were similar, but their relative abundances were different. The dominant flora of common species of P. tatarinowii was Bacillus, most species of this genus could improve the growth environment of plants to some extent, and enhance the ability of plant resistance to adversity and disease. Therefore, the root endophytic bacteria of P. tatarinowii, like those of Z. insignis and other plants, improve the rhizosphere soil fertility to a certain extent.

Key words: endemic plants of China, Pteroceltis tatarinowii, endophytic bacteria, rhizosphere soil, fertility change