Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation

doi:10.11923/j.issn.2095-4050.cjas2023-0220

Abstract

Abstract:

In order to understand the regulation factors that may be involved in gonadal differentiation of P. Procambarus, in this study, the proteome of exosomes in the hemolymph before and after androgenic gland ablation of P. clarkii was sequenced and analyzed. The results showed that a total of 469 proteins were identified in hemolymph exosomes, and 98 differentially expressed proteins were screened. Among which two differentially expressed proteins, 14-3-3 zeta and cell division cycle protein 27 homolog, may be involved in the sex regulation of P. clarkii.

Key words: Procambarus clarkii, androgenic gland, exosomes, proteome

YANG Siqi, WANG Qishuai, LI Yanhe. Differential Proteomic Analysis of Exosomes in Hemolymph of Procambarus clarkii Before and After Androgenic Gland Ablation[J]. Journal of Agriculture, 2024, 14(11): 56-63.

Figures/Tables 7

References 26

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蛋白ID	蛋白名称	序列长度/aa	备注
TRINITY_DN46360_c2_g2	Vitellogenin（卵黄蛋白原）	1592	H组有， Q组没有的蛋白
TRINITY_DN45848_c0_g1	Neural-cadherin（神经钙粘蛋白）	3003	H组有， Q组没有的蛋白
TRINITY_DN36934_c0_g1	Myosin-8（肌球蛋白-8）	1002	H组有， Q组没有的蛋白
TRINITY_DN31439_c0_g3	Teneurin-m（跨膜蛋白-m）	2552
TRINITY_DN39238_c5_g6	Apolipophorins（载脂蛋白）	1771
TRINITY_DN41980_c0_g3	Glycogen debranching enzyme（糖原脱支酶）	1632
TRINITY_DN46185_c1_g1	Twitchin（颤搐蛋白）	6121	Q组有， H组没有的蛋白
TRINITY_DN39783_c1_g2	Zinc finger homeobox protein 3（锌指同源盒蛋白3）	2320
TRINITY_DN30023_c2_g2	Zinc finger protein 423 homolog（锌指蛋白423同源物）	1171
TRINITY_DN33875_c0_g2	Agrin（集聚蛋白）	1575
TRINITY_DN39770_c1_g1	Histone-lysine N-methyltransferase SETD2（组蛋白赖氨酸N甲基转移酶SETD2）	1105
TRINITY_DN35993_c1_g1	RB1-inducible coiled-coil protein 1（RB1感应线圈蛋白1）	1464

蛋白ID	蛋白名称	序列长度/aa	备注
TRINITY_DN46360_c2_g2	Vitellogenin（卵黄蛋白原）	1592	H组有， Q组没有的蛋白
TRINITY_DN45848_c0_g1	Neural-cadherin（神经钙粘蛋白）	3003	H组有， Q组没有的蛋白
TRINITY_DN36934_c0_g1	Myosin-8（肌球蛋白-8）	1002	H组有， Q组没有的蛋白
TRINITY_DN31439_c0_g3	Teneurin-m（跨膜蛋白-m）	2552
TRINITY_DN39238_c5_g6	Apolipophorins（载脂蛋白）	1771
TRINITY_DN41980_c0_g3	Glycogen debranching enzyme（糖原脱支酶）	1632
TRINITY_DN46185_c1_g1	Twitchin（颤搐蛋白）	6121	Q组有， H组没有的蛋白
TRINITY_DN39783_c1_g2	Zinc finger homeobox protein 3（锌指同源盒蛋白3）	2320
TRINITY_DN30023_c2_g2	Zinc finger protein 423 homolog（锌指蛋白423同源物）	1171
TRINITY_DN33875_c0_g2	Agrin（集聚蛋白）	1575
TRINITY_DN39770_c1_g1	Histone-lysine N-methyltransferase SETD2（组蛋白赖氨酸N甲基转移酶SETD2）	1105
TRINITY_DN35993_c1_g1	RB1-inducible coiled-coil protein 1（RB1感应线圈蛋白1）	1464

编号	描述	分类
K16197/K03350	Oocyte meiosis（卵母细胞减数分裂）	KEGG通路
K03350	Progesterone-mediated oocyte maturation（孕激素介导的卵母细胞成熟）	KEGG通路
GO:0008585	Female gonad development（雌性性腺发育）	GO term
GO:0046545	Development of primary female sexual characteristics（初级雌性性征发育）
GO:0008406	Gonad development（性腺发育）
GO:0007548	Sex differentiation（性分化）
GO:0045137	Development of primary sexual characteristics（初级性征的发展）
GO:0046660	Female sex differentiation（雌性性分化）
GO:0007565	Female pregnancy（雌性怀孕）
GO:0048608	Reproductive structure development（生殖结构发育）
GO:0061458	Reproductive system development（生殖系统发育）
GO:0008103	Oocyte microtubule cytoskeleton polarization（卵母细胞微管细胞骨架极化）
GO:0060180	Female mating behavior（雌性交配行为）
GO:0007309	Oocyte axis specification（卵母细胞轴规格化）
GO:0007308	Oocyte construction（卵母细胞的构建）
GO:0001541	Ovarian follicle development（卵泡发育）
GO:0030716	Oocyte fate determination（卵母细胞命运决定）
GO:2000242	Negative regulation of reproductive process（生殖过程的负调控）
GO:0048599	Oocyte development（卵母细胞发育）
GO:0035112	Genitalia morphogenesis（生殖器形态发生）
GO:0009994	Oocyte differentiation（卵母细胞分化）
GO:0046661	Male sex differentiation（雄性性分化）
GO:0090598	Male anatomical structure morphogenesis（雄性解剖结构的形态发生）
GO:0007314	Oocyte anterior/posterior axis specification（卵母细胞前/后轴规范化）
GO:0046546	Development of primary male sexual characteristics（初级雄性性征的发展）
GO:0032355	Response to estradiol（对雌二醇的反应）
GO:0048515	Spermatid differentiation（精细胞分化）
GO:0019098	Reproductive behavior（生殖行为）
GO:0007283	Spermatogenesis（精子生成）
GO:0045172	Germline ring canal（生殖系环管）

编号	描述	分类
K16197/K03350	Oocyte meiosis（卵母细胞减数分裂）	KEGG通路
K03350	Progesterone-mediated oocyte maturation（孕激素介导的卵母细胞成熟）	KEGG通路
GO:0008585	Female gonad development（雌性性腺发育）	GO term
GO:0046545	Development of primary female sexual characteristics（初级雌性性征发育）
GO:0008406	Gonad development（性腺发育）
GO:0007548	Sex differentiation（性分化）
GO:0045137	Development of primary sexual characteristics（初级性征的发展）
GO:0046660	Female sex differentiation（雌性性分化）
GO:0007565	Female pregnancy（雌性怀孕）
GO:0048608	Reproductive structure development（生殖结构发育）
GO:0061458	Reproductive system development（生殖系统发育）
GO:0008103	Oocyte microtubule cytoskeleton polarization（卵母细胞微管细胞骨架极化）
GO:0060180	Female mating behavior（雌性交配行为）
GO:0007309	Oocyte axis specification（卵母细胞轴规格化）
GO:0007308	Oocyte construction（卵母细胞的构建）
GO:0001541	Ovarian follicle development（卵泡发育）
GO:0030716	Oocyte fate determination（卵母细胞命运决定）
GO:2000242	Negative regulation of reproductive process（生殖过程的负调控）
GO:0048599	Oocyte development（卵母细胞发育）
GO:0035112	Genitalia morphogenesis（生殖器形态发生）
GO:0009994	Oocyte differentiation（卵母细胞分化）
GO:0046661	Male sex differentiation（雄性性分化）
GO:0090598	Male anatomical structure morphogenesis（雄性解剖结构的形态发生）
GO:0007314	Oocyte anterior/posterior axis specification（卵母细胞前/后轴规范化）
GO:0046546	Development of primary male sexual characteristics（初级雄性性征的发展）
GO:0032355	Response to estradiol（对雌二醇的反应）
GO:0048515	Spermatid differentiation（精细胞分化）
GO:0019098	Reproductive behavior（生殖行为）
GO:0007283	Spermatogenesis（精子生成）
GO:0045172	Germline ring canal（生殖系环管）

蛋白ID	蛋白名称	序列长度/aa	分子量/ kDa	非标记定量信号强度-Q	非标记定量信号强度-H	Ratio(H/Q)	表达量上调或下调
TRINITY_DN31302_c2_g2	14-3-3 zeta	206	23.527	28304000	107000000	3.780384398	上调
TRINITY_DN37497_c4_g2	Cell division cycle protein 27 homolog	806	89.33	8123000000	4587100000	0.564705158	下调