Abstract:

In order to gain knowledge of genetic diversity of wheat varieties/lines resistance to wheat aphids, on the basis of field insect plot appraisal, 47 wheat varieties/lines with different degrees of resistance to wheat aphids were studied by 18 SSR markers to explore genetic diversity of wheat aphids. 18 SSR markers were screened and a total of 99 allelic variants were detected in 47 wheat varieties ranged from 1 to 10 alleles per marker with an average of 5.21. The mean of alleles of high resistance insect (HR) wheat varieties were 3.83, of resistance insect (R) wheat varieties were 3.88, of sense insect (S) wheat varieties were 4.06 and of high sense insect (HS) wheat varieties were 3.56. The PIC (Polymorphism information contents, PIC) for each polymorphic primer varied from 0 to 0.91 with an average of 0.64. PIC of the HR wheat varieties were an average of 0.60 (0-0.97), of the R wheat varieties were an average of 0.64 (0-0.99), of the S wheat varieties were an average of 0.64 (0.14-0.85) and of the HS wheat varieties were an average of 0.67 (0-0.81). The mean genetic distance (GD) between 47 varieties was 0.65 varied from 0.33 to 0.94. The GD between HR variety was 0.59 varied from 0.39 to 0.77; The GD between R variety was 0.62 varied from 0.39 to 0.83. The GD between S varieties was 0.62, varied from 0.37 to 0.83. The GD between HS variety was 0.64, varied from 0.37 to 0.84. Based on 0.67 of GD, 47 varieties were clustered into seven groups. Resistant insect wheat variety ‘Linyuan 95-5322’ was a groups by oneself. It was explained that there was a further genetic relationship between ‘Linyuan 95-5322’ and other variety, and ‘Linyuan 95-5322’ could be used to resistant insect parent.