Abstract: The aim of this study is to clone insecticide target glutamate-gated chloride channel (GluCl1) gene from Tetranychus cinnabarinus and analyze its sequence character, using a targeted degenerate PCR and RACE strategy. Full-length of GluCl1 gene was obtained. The cDNA sequence of 1856 bp was determined, which contains an open reading frame encoding an GluCl1 precursor of 455 amino acid residues, with a GenBank accession No. KC543353. The predicted signal peptide of T. cinnabarinus was 25 amino acid residues long at its N-terminal, and GluCl1 had four transmembrane domains. Alignment analysis showed that, the GluCl1 gene of T. cinnabarinus were homologous with those of others belonged to Acarina, especially with those of T. urticae. More interestingly, the amino acid site G314 in the 3rd transmembrane domain of GluCl1 of T. cinnabarinus sensitive population was identical to that of T. urticae sensitive population G323, but which was displaced by D323 in the GluCl1 gene of T. urticae insecticide-resistant population. This indicated that this mutation was related with the resistance of T. urticae to abamectin. This study laid the solid foundation for establishing an in vitro acaricide screening system targeted to glutamate-gated chloride channel of T. cinnabarinus in the future.