Current issue

• 2013 Vol.3 No.6 Published:20 June 2013

  The Establishment of Sorghum Cell Suspension Lines with Shoot Tips and Immature Inflorescences 2013, 3 (6):  1-3.  Abstract + ( 1110 )   PDF ( 1173KB )   ( 1136 ) The establishment of an effective and reliable cell suspension culture method is of great significance for protoplast culture and transgenic research. With shoot tips and immature inflorescences as explants, we carried out research on the establishment of sorghum cell suspension lines by the three ways of callus shake culture, homogenate collection culture and whole explants direct culture. The results indicate that the immature inflorescence is the better explants for cell suspension culture of sorghum, compared with shoot tips. All the three culture methods of immature inflorescences are applicable. Among them, callus shake culture is the most stable and reliable method. Although the other two methods have less stability and effectiveness, but they enjoy better comprehensive cell growth status including higher cell propagation capability. The results also show that the shoot tip callus shake culture is also a reliable preparation way of cell suspensions, the shoot tips homogenate culture is less reliable, while whole explants direct culture gave poor result and should not be used.

  Analysis on the Combining Ability of Quality Characteristics for New Glutinous Sorghum Male Sterility Lines 2013, 3 (6):  4-7.  Abstract + ( 1155 )   PDF ( 1139KB )   ( 1149 ) In order to evaluate the new glutinous sorghum male sterility lines scientifically and accurately, provide excellent parent materials for quality improvement of glutinous sorghum, three sterile lines and three restorer lines were chosen, 9 hybrid combinations were matched by incomplete diallel crosses method in this study. The qualities about protein, fat, starch, tannic were analyzed by combining ability and genetic effects. The results showed that, in the three new glutinous sorghum male sterility lines, 11476A2 exhibited higher general combining ability in starch and fat. 11486A2 only presented higher general combining ability in protein. 11494A2 showed higher general combining ability in tannic. The order of ratio of additive genetic variance to genotype variance was: protein > starch > tannic > fat. Bying glutinous sorghum breeding techniques target, 11476A2 presented high starch combining ability, and low protein combining ability, so that it may be a good glutinous sorghum parent system.

  Effects of DPC and Plant Pruning with Topping on Cotton Yield and Fiber Quality 2013, 3 (6):  8-12.  Abstract + ( 1042 )   PDF ( 1425KB )   ( 1628 ) In order to study the feasibility of retaining nutrition branch with non-topping in Yangtze River region and the interaction effect of pruning with topping and DPC, the field experiments were conducted in 2011 and 2012 to study the effects of DPC and pruning with topping on cotton yield, yield components and fiber quality at the Lukou farmland in Ezhou, Hubei Province. The experimental material was hybrid cotton H318 variety. The results showed that retaining nutrition branch and non-topping could add the total boll number per square meter, but decreased the boll weight, the lint yield and boll number per square meter had significant differences in 2012, and increased 14.3% and 13.3%, respectively. Non-pruning and non-topping was no effect on yield, sometimes it could increase cotton yield, and the lint percentage was not affected by pruning and topping. Spraying DPC increased boll weight, but decreased boll number, and it had not a significant effect on lint yield, sometimes it decreased lint percentage. Pruning and topping and spraying DPC didn’t affect fiber length, they had a significant effect on uniformity index and micron value, respectively. Cotton had a bigger uniformity index if it was pruned and topped, and it had a better fiber length and specific breaking strength after spraying DPC. So, a conclusion can be got that retaining nutrition branch and non-topping had a potential to improve yield, and spraying DPC should be applied according to the cotton growing status.

  Grey Relational Evaluation Study in Ear Traits and Yield of Different Maize Varieties 2013, 3 (6):  13-16.  Abstract + ( 1088 )   PDF ( 1391KB )   ( 1252 ) In order to overall evaluated sixteen maize varieties’ suitability in local agricultural ecological circumstance, we studied nine corn ear traits and yield of sixteen maize varieties with the grey relational analysis method through field experiment collected ear traits and yield factors data. Finally, we established a varieties assessment model to be used in sixteen maize varieties comprehensive comparison. The results showed that, three biggest grey relation traits were ear diameter and grain rate, row grains and grain rate, per ear grains weight and per ear weight. The grain rate, row grains, 100-seed weight had most correlation degree with yield. Through the varieties assessment model, ‘Xundan29’, ‘Xianyu335’, ‘Jindan55’, ‘Zhengdan958’, ‘Xundan26’ showed the best advantage. The weighted grey correlation degree assessment model was similar with equal weight assessment model. To breed new maize varieties, we first assured screening optimal grain rate, row grains and 100-seed weight, following coordination with residual ear traits in maize yield breeding practice. The grey relation analysis may play as a data statistic method in maize new varieties introduction and cultivation extension praxis, it will also better service in local agricultural production.

  The Influences of Different Nitrogen to the Properties of Soybean Production on ‘Henong60’ cheng 2013, 3 (6):  17-19.  Abstract + ( 1216 )   PDF ( 1098KB )   ( 1091 ) In order to define the relations between the nitrogen level and soybean production, and determine the best level of nitrogen. By means of narrow row close planing mode and different nitrogen levels, with the ‘Henong60’ as the research object, discussed the influence of different nitrogen levels on the yield factor and plant trait of soybean, and analyzed the economic benefit. The results showed that: the higest yield was 273.4 g/m2 of N2P1K1 treatment, nitrogen was also evident effects on plant height, the highest plant height was 83.4 cm of N3P1K1 treatment. The height of bottom pods also increased at 36.1 cm. Therefore, when the content of phosphate and potash fertilizer were fixed in a certain range, with more nitrogen helped to increase yield and economic benefit. From analysiing production and economic benefit, the experiment N2P1K1 was the best treatment.

  Cloning and Characterization of Full-length Glutamate-gated Chloride Channel Gene from Tetranychus cinnabarinus 2013, 3 (6):  20-26.  Abstract + ( 1202 )   PDF ( 1816KB )   ( 2139 ) The aim of this study is to clone insecticide target glutamate-gated chloride channel (GluCl1) gene from Tetranychus cinnabarinus and analyze its sequence character, using a targeted degenerate PCR and RACE strategy. Full-length of GluCl1 gene was obtained. The cDNA sequence of 1856 bp was determined, which contains an open reading frame encoding an GluCl1 precursor of 455 amino acid residues, with a GenBank accession No. KC543353. The predicted signal peptide of T. cinnabarinus was 25 amino acid residues long at its N-terminal, and GluCl1 had four transmembrane domains. Alignment analysis showed that, the GluCl1 gene of T. cinnabarinus were homologous with those of others belonged to Acarina, especially with those of T. urticae. More interestingly, the amino acid site G314 in the 3rd transmembrane domain of GluCl1 of T. cinnabarinus sensitive population was identical to that of T. urticae sensitive population G323, but which was displaced by D323 in the GluCl1 gene of T. urticae insecticide-resistant population. This indicated that this mutation was related with the resistance of T. urticae to abamectin. This study laid the solid foundation for establishing an in vitro acaricide screening system targeted to glutamate-gated chloride channel of T. cinnabarinus in the future.

  Breed Semi-dwarf, Resistant High Density and Super High-yielding Soybean Variety 'Henong 60' Using American Dwarf Source as Hybridization Paternal 2013, 3 (6):  27-30.  Abstract + ( 1242 )   PDF ( 1512KB )   ( 1990 ) In order to semi dwarf soybean new variety breeding to promote cultivation technique of narrow row and thick planting, using United States half-dwarf germplasm resources resistant to high density, high-oil soybean germplasm in Heilongjiang Province and Hobbit 'North 11th' preparation of hybrid combination, researching for semi dwarf compact planting of soybean germplasm, improved semi dwarf soybean new variety 'Farm 60th'. The results showed that, 'Farm 60th', with half-dwarf compact planting, plant height 50-60 cm, under the conditions of narrow row and thick planting suitable density was from 500 thousands plants to 600 thousands plants. With 5467.95 kg/hm2 high yield potential, 22.25% fat content, disease resistance, and other distinctive advantages, had a good prospect of application and extension.

  Comparative Study on Main Disease of Kasugamycin WP and Conventional Pesticide Japonica 2013, 3 (6):  31-33.  Abstract + ( 1217 )   PDF ( 1176KB )   ( 1234 ) In order to carry out of the Kasugamycin WP and conventional chemical pesticides, a comparative study in the prevention and treatment of major diseases of the japonica. Separate administration of Kasugamycin WP and conventional chemical pesticides in rice growth period, and then study the effect of Kasugamycin WP and conventional chemical pesticides to control the main diseases of the japonica. The results showed that, 2% Kasugamycin WP in the blast and false smut control effect than other agents, sheath blight control effect below to take the enemy's steady 75% WG and 300 g/L Armure EC. The comparison of applied agent cell yield, 2% Kasugamycin WP slightly lower than take the enemy's steady 75% WG and 300 g/L Armure EC. Kasugamycin WP in japonica disease control effect as much as conventional chemical pesticides, since the Kasugamycin WP lower on the environment and human and animal hazards, with a high use value.

  Relationship between plant rhizosphere microbiome and biological control against soil-borne disease 2013, 3 (6):  34-38.  Abstract + ( 1165 )   PDF ( 1077KB )   ( 1414 ) The rhizosphere microbiome, also referred to as the second genome of the plant, is crucial for plant health. Thus, it is the most important resource for biological control agents screening. Here we summarized the recent research development on relationship between plant and the rhizosphere microbiome: including how a plant shapes its rhizosphere microbiome actively; how the root microbiome act on the “rescue” mechanism; and how the biological control agents and the native microorganisms act on each other.

  A review on occurrence and biological control of Mononychellus tanajoa 2013, 3 (6):  39-44.  Abstract + ( 1274 )   PDF ( 1189KB )   ( 1244 ) Mononychellus tanajoa is a severe pest, owing to its damage to the growth and yield to the cassava，it has been considered a greater threat to the cassava industry. The paper summarized the propagation，damage mechanisms，influencing factors of the damage to the cassava and generalized the researches of existing of the biological control studies. Finally，further studies of Mononychellus tanajoa on the physiological and biochemical characteristics of cassava，biological control strains infecting mechanism to Mononychellus tanajoa，as well as the relationship with the environment, suitable natural enemies collocation to biological control were put forward.

  The Historical Change of Plant Species Caused by Living Habitant’s Variation 2013, 3 (6):  49-53.  Abstract + ( 1123 )   PDF ( 1859KB )   ( 1371 ) Abstract：With rapid economic and social development, urbanization rising population growing, the city changes rapidly brings us a convenient life but it also brings a series of environmental problems. We are plagued with them caused by climate change. Base on the analysis of the living environment, reviewing of historical literature and poetries, comparing the plant growth habits and distributions, the dynamic change of plant species are summarized with time series. The plant succession is explored with the perspective of historical development and climate characters.

  The Production Procedure of Potted Flower 0rnamentaI Pineapple 2013, 3 (6):  54-56.  Abstract + ( 1226 )   PDF ( 1176KB )   ( 1180 ) As one of the main ornamental plant,ornamental pineapple is more and more popular on consumers.This study elaborates the production of greenhouse & facilities, cultivation techniques,pest control,packaging of potted ornamental pineapple.in order to provide a technical guidance to production．

  Discussion on the Comprehensive Utilization and Industrialization of Eucommia ulmoides 2013, 3 (6):  57-60.  Abstract + ( 1000 )   PDF ( 1333KB )   ( 1791 ) Eucommia ulmoides, the Chinese endemic plant, belongs one of second class conservation plant of China. It has significantly medical and sanitarian value. Chemical compositions and pharmacological effects of Eucommia ulmoides were introduced in this review, as well as the present comprehensive utilization of Eucommia ulmoides on medicine, health production, feed additive, gutta-percha and lumber were analyzed. Finally, the necessity of Eucommia ulmoides industrial development was issued according to its latest bases and problems. Then tactics and techniques on Eucommia ulmoides industrial development were discussed. The research of key technology played an important role in their practice.

  Research Progress on Genetic Diversity of Germplasm Resources of Onion (Allium cepa L.) 2013, 3 (6):  61-63.  Abstract + ( 1208 )   PDF ( 1042KB )   ( 1629 ) Study on genetic diversity of germplasm resources of onion helps accelerate the onion breeding process, improve breeding efficiency and quality. Several aspects of research for genetic diversity, including origin and classification, morphological markers, cytological markers, biochemical markers, and DNA markers were reviewed in the paper.

  Development Present Situation and Consideration of Southern Type of Poplar Industry in Siyang 2013, 3 (6):  64-67.  Abstract + ( 1060 )   PDF ( 1470KB )   ( 1242 ) Introduction, demonstration, promotion of southern type of poplar 41 years in Siyang, average annual planting poplar 1.5 million, poplar into existing forest area of 36600 hectare, processing of poplar annual output value of 10 billions yuan, but with the development of poplar industry highlights resources, unbalanced development, irrational industrial structure and contradiction. In a comprehensive analysis of Siyang County, southern type poplar development history, present situation and the problems, some countermeasures and suggestions on the future development of southern type of poplar industry in Siyang was put forward.

  Studies on Enzymatic Properties of Laccase from Hybrid Strains of Flammulina velutipes 2013, 3 (6):  68-71.  Abstract + ( 1062 )   PDF ( 1554KB )   ( 1340 ) Abstract: L7 as experimental material is hybrid strains of Flammulina velutipes. The native—PAGE analysis showed that it possessed three kinds of laccase isoenzymes. By using spectrophotometry and choosing ABTS[2,2’—Azino-bis(3一ethy lbenzothia— z0line一6—sulfonicacid)]，the activity of laccase can be determined, the results show that: the appropriate determination of a wavelength 410 nm,system pH4.5, reaction temperature 45℃.

  Tilapia Breeding Situation and Development Prospect of Tibet 2013, 3 (6):  72-74.  Abstract + ( 1103 )   PDF ( 1038KB )   ( 1516 ) The development histories of tilapia breed at Tibet were surveyed. The development tendencies of tilapia consumption markets at Tibet were analyzed and the status and existing problems of tilapia industrialized management in Tibet were discussed. The major advantages of tilapia in Tibet were catalogued. And put forward the further development of Tibet tilapia breeding potential.

  Research on the Test Method of Average Heat Transfer Coefficient for Passive Solar Greenhouses 2013, 3 (6):  75-78.  Abstract + ( 1090 )   PDF ( 1613KB )   ( 1253 ) Using lumped parameter technique, a passive solar greenhouse with hot water system to adjust the warm performance was regarded as the plate collector.Using the similar test method to determine the thermal performance of domestic solar heaters, the greenhouse heat balance and heat loss and average heat transfer coefficient had analyzed. In view of a greenhouse, the average heat transfer coefficient was tested and calculated. The results showed that the glass greenhouse, covering an area of 45.09 m2 ground for reinforced concrete roof, without soil and crop, the average heat transfer coefficient was 5.3 W/(m2?℃). It has a reference and the practical value to the greenhouse thermal properties analysis.