Abstract: Abstract: To solve Vitis quinquangularis Rehd. using traditional hard branch cuttage breeding method, the problem of low survival rate, the project team through continuous practice and improvement, using earth micropropagation as nursery seedling technology formed a complete " Yeniang No.2" Hermaphrodite Flower Vitis quinquangularis Rehd. breeding system, this article from the somaclone provisonal planting cultivation, transformation of sand bed - twigs sand bed cutting provisonal planting - take root seedling nutrition cup provisonal planting three-step provisonal planting seedling, provisonal planting, fertilization, provisonal planting pest control management four aspects this paper expounds the " Yeniang No.2" Hermaphrodite Flower Vitis quinquangularis Rehd. seedling comprehensive technology, using this technology, can be a lot of production, supply high quality seedlings in a short time.