Abstract: The study aims to prepare a water-soluble calcium fertilizer which containing calcium glycinate, in order to make calcium absorbed by plant more quickly and efficiently. Glycine and calcium nitrate tetrahydrste were used as the raw materials, and orthogonal experimentation were used to investigate the effect of raw materials ratio, reaction temperature, solution pH and the reaction time on the reaction results. The calcium content was measured by EDTA titration, and the chelating rate was measured by alcohol precipitation. The optimum technological conditions were that mole ratio of glycine to calcium nitrate tetrahydrate was 1.5:1, reaction temperature was 50℃, solution pH was 5.5, and reaction time was 1 h. Under these conditions, the total calcium content was 103.38 g/L, chelated calcium was 58.91 g/L, and chelating rate was 56.98%. By this method, a water-soluble calcium fertilizer possessed of appropriate total calcium content can be gotten, and it is in line with the agricultural industry standards of high chelating rate type liquid calcium fertilizer.