Abstract:

The research as the transgene system establishment, the agricultural bacillus lesds transformed the research to lay the foundtion which has further provided the theory basis and the technical guarantee for the genetic engineering improvement of the buckwheat biosynthesis. Selecting the buckwheat which is windsor sweet buckwheat for establishing regeneration system and antibitic screening system, We know explants are cotyledon and hypocotyl. The basic culture medium is called MS. We can add into the different Auxin and cytokinin under the different condition Auxin and cytokinin under the different condition, we studied Jiujiang bitter buckwheat of the seed leaf and under the hypocotyl injuries the organization the induction as well as does not decide the bud differentiation and the growth situation we can establish the best regeneration system through researching the different explants, as the transgene system establishment has further provided the theory basis. The research indicated that The windsor sweet buckwheat seed leaf produces injuries the organization the best culture medium is MS + 2,4-D 2 mg/L + 6-BA 0.4 mg/L. The induction bud's best culture medium is MS + 6-BA 1.5 mg/L + IBA 0.3 mg/l.The next hypocotyl induction injuries the organization the best culture medium is MS + 2,4-D 3 mg/L + 6-BA 0.5 mg/l, the induction bud's best culture medium is MS + 6-BA 0.05 mg/L + IBA 0.1 mg/L. The rooting induction the best culture medium is 1/2 MS.