Carried out for seed corn fertilizer law to provide a theoretical basis for the promotion of balanced fertilization technology, and improve seed production, reducing the adverse effects on the ecological environment of the blind fertilization. The characteristic of fertilizer requirement of seed production corn in Hexi Corridor was studied based on the multi-site experiments under high, mid and low fertilizing levels. The results showed that, the average seed yield of ZhengDan958 and ShengDan16 achieved 7744.5 kg/hm2 at sufficient quantities of fertilizer conditions; The demands for N, P2O5 and K2O for production of 100 kg of corn seeds were 2.653 kg, 1.115 kg and 3.449 kg respectively, in a proportion of 1:0.42:1.30. The dry matter accumulation process reached to 0.11%, 4.0%, 21.3%, 50.4%, 94.9% and 100% at the periods of trefoil, hexaphyllous, jointing, tasseling, grain filling and mature stage. The N, P and K accumulation rate were 43.6%, 37.8%, 58.3% during the seedling to jointing stage, 22.1%, 27.1%, 18.3% during the jointing to tasseling stage, 26.3%, 19.9%, 14.5% during the tasseling to grain filling stage, and 8.0%, 15.7%, 8.9% during the grain filling to mature stage respectively. The nutrient absorption peak occurred in the jointing stage. Base fertilizer should be emphasized in seed corn production field, while nitrogen and potassium fertilizers should be top dressed in the jointing stage and nitrogen and phosphorus fertilizers slightly dressed in the tasseling stage, so as to meet the nutrient demands of the seed production. The results showed that the mid fertilizing level maximized the seed production potential completely, up to 516.3 kg per 666.7 m2, while excessive fertilizer applying had an indistinctive effect on yield.