Abstract:

The composition of fermented broth and fermentation conditions were optimized by monofactorial and orthogonal experiment respectively. Combination of the C, N sources, content of the C, N sources and NaCl, had different effect on the antagonistic activity of bacteria GF-16. The broth composed of 1% amylaceum, 1.5% Huang bean cake powder and 0.5% NaCl was better for antagonistic effect of GF-16. Through the study of a few fermentation conditions of antagonistic bacteria GF-16, The most preferable fermentation condition was fermented for 36h, pH7, 7%inoculation, 110mL liquid in 500mL conical flaskand, 180r/min, 28oC.