Abstract:

The regulating and controlling roles of chloride and zinc ions to swine sperm calcium channel and their mechanism were studied. Semen from healthy boar were filtered with gauze and divided into three groups, each group having 6 repeats. The group 1 semen was not added anything; the group 2 and 3 semen were added NaCl and ZnSO4 solution respectively. Being mixed and placed 2.5 h, various parameters of three experimental semen were measured. Experimental results showed that: (1)supplementation of optimum chlorine ions or zinc ions in semen could stimulate migration of calcium ions from semal plasm to sperm, thus calcium ion concentration in sperm rised significantly (P＜0.05), calcium ion concentration in semal plasm decreases slightly; (2)supplementation of chlorine or zinc ions in semen could strengthen sperm motility highly significantly (P＜0.01); (3) after supplementation of zinc ions in semen，the activities of AKP, CA and AC and the concentration of cAMP in sperm increased highly significantly (P＜0.01), the activities of AKP and AC in semal plasm rised highly significantly (P＜0.01), the concentration of cAMP in semal plasm increased significantly(P＜0.05); (4) after supplementation of chlorine ions in semen, the activities of AKP and AC in semal plasm rised highly significantly (P＜0.01), the concentration of cAMP in semal plasm increased significantly (P＞0.05). These results suggested that supplementation of optimum zinc or chlorine ions in boar semen might up-regulate the function of sperm calcium channel, and could improved the activities of AKP, CA and AC and the concentration of cAMP in semen, strengthen sperm motility.